Karyotype analysis in grass pea (Lathyrus sativus L.)
DOI:
https://doi.org/10.59797/jfl.v25i1.1206Keywords:
Chromosome, Constriction, Grass pea, KaryotypeAbstract
In order to understand the chromosome morphology and structural variation, somatic chromosomes of nine accessions of grass pea (Lathyrus sativus L.) viz., ‘Pusa 24’, ‘Ratan’, ‘Pratik’, ‘IC120455’, ‘IC120500’, ‘IC120505’, ‘IC345392’, ‘IC345401’, and ‘IC345403’ were studied using young root tips. The study reveale d variati on not only in physical dimension and morphology of the chromosomes but also in the position of the primary and secondary constrictions. Although all accessions were dipl oid (2n=14), variati ons in karyotype formula, chromosome length, total haploid complement, arm ratio and centromeric index were observed. The secondary constriction was observed in at least one of the metacentric chromosomes of ‘Pusa 24’, ‘IC120500’, ‘IC120505’, ‘IC345392’ and ‘IC345403’. Telocentri c chromosomes were absent in all the accessions while variable numbers of sub-metacentri c and acrocentric chromosomes we re obse rved in these accessi ons. The se chromosomes differed in their lengthwise posi tions in the karyotype. Total length of the largest chromosomes did not differ significantly among accessions. However, chromosomes with satellites were longer. Total length of smallest chromosome was variable among the acce ssi ons. The total hapl oid chromosome length (TCL) varied from 5.11 μm in ‘IC345403’ to 7.30 μm in ‘IC345401’. The arm ratio and centromeric index value ranged from 1.21 in ‘IC120455’ to 1.44 in ‘IC120505’ and 40.94 in ‘IC120505’ to 45.19 in ‘IC120455’, respectively. These resul ts clearly showed structural variation in karyotype at genotypic level in grass pea.
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