Synteny relationships among the linkage groups of chickpea (Cicer arietinum L.)
DOI:
https://doi.org/10.59797/jfl.v24i2.1289Keywords:
Ascochyta rabiei, BSA, Chickpea, ICCM markers, STMS markersAbstract
Ascochyta blight (AB) caused by Ascochyta rabiei L. is a devastating disease of chickpea world-wide. Resistant germplasm has been identified and an F2 mapping population of chickpea cultivars with contrasting disease reactions to Ascochyta rabiei L. was used to identify the markers linked to the disease using STMS and ICCM markers. STMS markers showed 40% polymorphism between the parents. Bulked segregant analysis (BSA) was performed to identify markers linked to the AB resistance gene. Of the 252 markers, only 10 were found to be possibly linked. MAPMAKER version 3.0 gave the linkage map of LG5 region of the chickpea genome spanning a length of 275.2 cM. The AB resistance gene was present on LG5 flanked by the markers TA 42 and TR 35, with a distance of 45.0 cM and 38.6 cM, respectively. It was concluded that there was synteny in the linkage groups 1, 3, 5 and 7.
References
Arumuganathan K and Earle ED. 1991. Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9: 208-218.
Cho YG, Ishii T, Temnykh S, Chen X, Lipovich L, McCouch SR, Park WD, Ayres N and Cartinhour S. 2000. Diversity of microsatellite derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). Theoretical and Applied Genetics 100: 713-722.
Croser JS, Ahmad F, Clarke HJ and Siddique KHM. 2003. Utilization of wild Cicer in chickpea improvement- progress, constraints andprospects. Australian Journal of Agricultural Research 54: 429-444.
Ferguson ME, Burrow MD, Schulze SR, Bramel PJ, Paterson AH, Kresovich S and Mitchell S. 2004. Microsatellite identification and characterization in peanut. Theoretical and Applied Genetics 108: 1064-1070.
Flandez-Galvez, Ford HR, Pang ECK and Taylor PWJ. 2003. An intraspecific linkage map of the chickpea (Cicer arietinum L.) genome based on sequence tagged microsatellite site and resistant gene analog markers. Theoretical and Applied Genetics 106: 1447-1456.
Gadish I and Zamir D. 1987. Differential zygotic abortion in an interspecific Lycopersicum cross. Genome 29: 156-159.
Gaitan Solis E, Duque MC, Edward KJ and Tohme. 2002. Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross species amplification in Phaseolus spp. Crop Science 42: 2128-2136.
He C, Poysa V. and Yu K. 2003. Development and characterization of simple sequence repeats (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars. Theoretical and Applied Genetics 106: 363-373.
Huttel B, Winter P, Weisind K, Chromosome W, Weigand F and Kahl G. 1999. Sequence tagged microsatellite markers for chickpea (Cicer arietinum L). Genome 42: 210-217.
Kazan K, Muehlbauer FJ, Weeden NF and Ladizinsky G. 1993. Inheritance and linkage relationships of morphological and isozyme loci in chickpea (Cicer arietinum L.). Theoretical and Applied Genetics 86: 417-426.
Kusmenoglu I. 1990. Ascochyta blight of chickpea: Inheritance and relationship to seed size, morphological traits and isozyme variation. M.Sc. Thesis, Washington State University, Pullman, USA.
Kusmenoglu I, Muehlbauer FJ and Kazan K. 1991. Inheritance of isozyme variation in ascochyta blight resistant chickpea lines. Crop Science 32: 121-127.
Lichtenzveig J, Scheuring C, Dodge J, Abbo S and Zhang HB. 2005. Construction of BAC and BIBAC libraries and their application for generation of chickpea (Cicer arietinum L.) map. Theoretical and Applied Genetics 110: 492-510.
Lyttle TW. 1991. Segregation distorters. Annual Review of Genetics 25: 511-557.
Mahendra Pal, Pandia R S, Kumar J and Singh B. 1999. Genetics of resistance to Ascochyta blight in chickpea. Indian Phytopathology 52: 403-407.
Michelmore RW, Paran I and Kesseli RV. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proceedings of Natural Academy of Sciences USA 88: 9828-9832.
Rakshit S, Winter P, Tekeoglu M, JuarezMunoz J, Pfaff T, BenkoIseppon AM, Muehlbauer FJ and Kahl G. 2003. DAF marker tightly linked to a major locus for Ascochyta blight resistance in chickpea (Cicer arietinum L.). Euphytica 132: 23-30.
Saghai Maroof HA, Soliman KM, Jorgensen AR, and Allard RW. 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of Natural Academy of Sciences USA 81: 8014-8018.
Santra DK, Tekeoglu M, Ratnaparkhe M, Kaiser WJ and Muehlbauer FJ. 2000. Identification and mapping of QTLs conferring resistance to Ascochyta blight in chickpea. Crop Science 40: 1606-1612.
Sethy NK, Shokeen B, Edwards KJ and Bhatia S. 2006. Development of microsatellite markers and analysis of intraspecific genetic variability in chickpea (Cicer arietinum L). Theoretical and Applied Genetics 112: 1416-1428.
Sharma YR, Singh G and Kaur L. 1995. A rapid technique for Ascochyta blight resistance in chickpea. International Chickpea and Pigeonpea Newsletter 2: 34-35.
Simon CJ and Muehlbauer FJ. 1997. Construction of chickpea linkage map and its comparison with maps of pea and lentil. Journal of Heredity 88: 115-119.
Spurthi NN, Hongyan Z, Varghese N, Datta S, Choi H, Horres R, Jungling R, Singh J, Kishor PBK, Sivaramkrishnan S, Hoisington DA, Kahl G, Winter P, Cook DR, Varshney RK. 2010. Integration of novel SSR and gene-based SNP marker loci in the chickpea genetic map and establishment of new anchor points with Medicago truncatula
genome. Theoretical and Applied Genetics 120: 1415–1441.
Udupa SM and Baum M. 2003. Genetic dissection of pathotype specific resistance to ascochyta blight resistance in chickpea (Cicer arietinum L.) using microsatellite markers. Theoratical and Applied Genetics 106: 1196-1202.
Udupa SM, Robertson LD, Weigand F, Baum M and Kahl G. 1999. Allelic variation at (TAA)n microsatellite loci in a world collection of chickpea (Cicer arietinum L.) germplasm. Theoretical and Applied Genetics 97: 299-307.
Voorripps R E. 2002. Map Chart: Software for the graphical presentation of linkage maps and QTLs. Journal of Heredity 93: 77-78.
Winter P, Benkoiseppon A M, Huttel B, Ratnaparkhe M, Tullu A, Sonnante G, Pfaff T, Tekeoglu M, Santra D, Sant V J, Rajesh P N, Kahl G and Mubhlbauer F J. 2000. A linkage map of chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from C. arietinum × C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5. Theoretical and Applied Genetics 101: 1155-1163.
Winter P, Ptaff T, Udupa SM, Huttel B, Sharma PC, Sahim S, Arreguin- Espinoza R, Weigand F, Muehlbauer FJ and Kahl G. 1999. Characterization and mapping of sequence tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Molecular Journal of General Genetics 262: 90-101.
Zamir D and Tadmor Y. 1986. Unequal segregation of nuclear genes in plants. Botanical Gazzette 147: 355-358.
