Genetic diversity analysis among local bean (Phaseolus vulgaris L.) cultivars using RAPD markers

Authors

  • NIRJA THAKUR Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India Author
  • SUBASH CHAND PARMAR Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India Author
  • AMARJIT K NATH Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India Author

DOI:

https://doi.org/10.59797/journaloffoodlegumes.v30i1.131

Keywords:

Common bean, Polymorphism, RAPD markers

Abstract

Random amplified polymorphic markers were used to evaluate genetic variations among nine bean cultivars (Phaseolus vulgaris L.) of Kinnaur district, Himachal Pradesh. Thirty five polymorphic RAPD markers generated 230 bands. Among these, 209 bands were polymorphic (90.86%) and 45 were unique. The primer OPA09 gave highest polymorphic information content (PIC) value of 0.90 while primer OPA 16 had lowest PIC value (0.58). Resolving power was 20 for OPA-11 and 106 for OPP-16. Jaccard’s coefficients based genetic similarity varied from 0.32-0.65. UPGMA based clustering patterns divided cultivars into two main groups and subgroups.

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Published

2024-06-02

Issue

Vol. 30 No. 1 (2017)

Section

Articles

How to Cite

Genetic diversity analysis among local bean (Phaseolus vulgaris L.) cultivars using RAPD markers. (2024). Journal of Food Legumes, 30(1), 1-5. https://doi.org/10.59797/journaloffoodlegumes.v30i1.131