In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp] using various explants

Authors

  • NANDHA ABHIJEETA K Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, India Author
  • MADARIYA RAJESH B Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, India Author

DOI:

https://doi.org/10.59797/journaloffoodlegumes.v30i1.133

Keywords:

Callus regeneration, In vitro, Pigeonpea

Abstract

An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties (BDN-2 and GT-101). Three different explants viz., leaf, hypocotyl and root excised from 15-20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. Shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine. Elongated shoots were transferred in MS + 1.0 mg/l IAA media for rooting. Rooted plants were transferred in pot for acclimatization.

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Published

2024-06-02

Issue

Vol. 30 No. 1 (2017)

Section

Articles

How to Cite

In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp] using various explants. (2024). Journal of Food Legumes, 30(1), 11-14. https://doi.org/10.59797/journaloffoodlegumes.v30i1.133