Significance and genetic diversity of SPAD chlorophyll meter reading in chickpea germplasm in the semi-arid environments
DOI:
https://doi.org/10.59797/jfl.v23i2.1411Keywords:
Breeding, Chickpea (Cicer arietinum L.), Genetic diversity, Mini-core collection, SPAD chlorophyll meter reading (SCMR)Abstract
The SPAD chlorophyll meter reading is a measurement of the leaf chlorophyll contents, viz., the nitrogen acquisition capability, and so it is often used to improve the yield through improved nitrogen status. The genetic diversity of the SCMR was investigated in the chickpea mini-core germplasm collection plus five control cultivars of chickpea (Cicer arietinum L.) (n = 216) of ICRISAT Genebank under field conditions during two consecutive post rainy seasons of 2005-06 and 2006- 07. A large genetic variability for SCMR was observed among the 216 chickpea accessions. The SCMR at 62 days after sowing was positively correlated with the seed yield under drought environments. The SCMR at the earlier or later growth stages or under irrigated environment was not related to yield under drought environment, indicating that the selections for SCMR in chickpea need to be done at about mid pod-fill stage under drought stress conditions. A known drought avoidant chickpea genotype, ‘ICC 4958’ that has prolific and deep rooting system also showed the best SCMR performances among the 216 chickpea germplasm. ‘ICC 4958’ can be a potential donor parent for both root systems and SCMR advantages. In addition, few other outstanding genotypes such as ‘ICC 1422’, ‘ICC 10945’, ‘ICC 16374’ and ‘ICC 16903’, with the higher SCMR, were also identified in this study. This genetic variability for SCMR in the mini core provides valuable baseline knowledge in chickpea for further progress on the selection and breeding for drought tolerance through nitrogen acquisition capability.
References
Ali M and Kumar S. 2003. Chickpea research in India. An over view. In: Masood Ali, Shiv Kumar, Singh NB (Eds), Chickpea research in India. Pp. 1-13. Indian Institute of Pulses Research, Kanpur, India.
Bänziger M, Edmeades GO and Lafitte HR. 1999. Selection for Drought Tolerance Increases Maize Yields across a Range of Nitrogen Levels. Crop Science 39: 1035-1040.
Bindu Madhava H, Sheshshayee MS, Shankar AG, Prasad TG and Udayakunar M. 2003. Use of SPAD chlorophyll meter to assess transpiration efficiency of peanut. In: Cruickshank AW, Rachaputi NC, Wright GC, Nigam SN, eds. Breeding of drought resistant peanuts. ACIAR Proceeding No. 112, Pp. 3-9. Australian Centre for International Agricultural Research, Canberra.
Bullock DG and Anderson DS. 1998. Evaluation of Minolta SPAD-502 chlorophyll meter for nitrogen management in corn. Journal of Plant Nutrition 21: 741-755.
Chandra S, Buhariwalla HK, Kashiwagi J, Harikrishna S, Rupa Sridevi K, Krishnamurthy L and Serraj R. 2004. Identifying QTL-linked markers in marker-deficient crops. 4th International Crop Science Congress, Brisbane, Australia.
Dua RP, Chaturvedi SK and Sewak S. 2001. Reference varieties of chickpea for IPR regime, Pp. 7. Indian Institute of Pulses Research, Kanpur, India.
Esechie HA and Al-Maskri AY. 2006. Relationship between SPAD-502 meter values and extractable chlorophyll in chickpea (Cicer arietinum L.). Research on Crops 7: 313-317.
El-Swaify SA, Pathak P, Rego TJ and Singh S. 1985. Soil management for optimized productivity under rainfed conditions in the semiarid tropics. Advances in Soil Science 1: 1-64.
Food and Agricultural Organization of the United Nations. 2009. FAOStatistical Databases. Available at http://faostat.fao.org/ FAO, Rome. Inada K. 1963. Studies on a method for determining deepness of green color and chlorophyll content of intact crop leaves and its practical applications. 1. Principle for estimating the deepness of green color and chlorophyll content of whole leaves. Proceedings of Crop Science Society of Japan 32: 157-162.
Inada K. 1985. Spectral ratio of reflectance for estimating chlorophyll content of leaf. Japanese Journal of Crop Science 54: 261-265.
Kamara AY, Kling JG, Ajala SO and Menkir A. 2001. Vertical rootpulling resistance in maize is related to nitrogen uptake and yield. Pp. 228-232. 7th Eastern and Southern African regional Maize Conference. Feb 11-15 2001.
Kashiwagi J, Krishnamurthy L, Upadhyaya HD, Krishna H, Chandra S, Vadez V and Serraj R. 2005. Genetic variability of drought-avoidance root traits in the mini-core germplasm collection of chickpea (Cicer arietinum L.). Euphytica 146: 213-222.
Kashiwagi, J, Krishnamurthy L, Crouch JH and Serraj R. 2006. Variability of root characteristics and their contributions to seed yield in chickpea (Cicer arietinum L) under terminal drought stress. Field Crops Research 95: 171-181.
Krishnamurthy L, Vadez V, Jyotsna Devi M, Serraj R, Nigam SN, Sheshshayee MS, Chandra S and Aruna R. 2007. Variation in transpiration efficiency and its related traits in a groundnut (Arachis hypogaea L.) mapping population. Field Crops Research 103: 189-197.
Kumar J, Haware MP and Simthson JB. 1985. Registration of four short-duration fusarium wil-resistant kabuli (garbanzo) chickpea germplasm. Crop Science 25: 576-577.
Ludlow MM and Muchow RC. 1990. Critical evaluation of traits for improving crop yields in water-limited environments. Advances in Agronomy 43: 107-153.
Nigam SN and Aruna R. 2008. Stability of soil analytical development (SPAD) chlorophyll meter reading (SCMR) and specific leaf area (SLA) and their association across varying soil moisture stress conditions in groundnut (Arachis hypogaea L.). Euphytica 160: 111-117.
Nageswara Rao RC, Talwar HS and Wright GC. 2001. Rapid assessment of specific leaf area and leaf N in peanut (Arachis hypogaea L.) using chlorophyll meter. Journal of Agronomy and Crop Science 189: 175-182.
Peng S, Garcia FC, Laza RC and cassmann KG. 1993. Adjustment for specific leaf weight improves chlorophyll meter’s estimation of rice leaf nitrogen concentration. Agronomy Journal 85: 987-990.
Richardson AD, Duigan SP and Berlyn GP. 2002. An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytol 153: 185-194.
Ryan JG. 1997. A global perspective on pigeonpea and chickpea sustainable production systems: Present status and future potential. In: Asthana AN, Ali M, eds. Recent Advantages in Pulses Research. Indian Society of Pulses Research and Development. Pp. 1-31. Indian Institute of Pulses Research (IIPR), Kanpur, India.
Saxena NP, Krishnamurthy L and Johansen C. 1993. Registration of a drought-resistant chickpea germplasm. Crop Science 33: 1424.
Silva MA, Jifon JL, Silva JAG and Sharma V. 2007. Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal Plant Physiology 19: 193-201.
Subbarao GV, Johansen C, Slinkard AE, Rao RCN, Saxena NP and Chauhan YS. 1995. Strategies for improving drought resistance in grain legumes. Critical Reviews in Plant Science 14: 469-523.
Takebe M and Yoneyama T. 1989. Measurement of leaf color scores and its implication to nitrogen nutrition of rice plants. Japan Agricultural Research 23: 86-93.
Turner FT and Jund MF. 1994. Assessing the nitrogen requirements of rice crops with a chlorophyll meter method. Australian Journal of Experimental Agriculture 34: 1001-1005.
Upadhyaya HD, Bramel PJ and Singh S. 2001. Development of a chickpea core subset using geographic distribution and quantitative traits. Crop Science 41: 206-210.
Upadhyaya HD and Ortiz R. 2001. A mini core subset for capturing diversity and promoting utilization of chickpea genetic resources in crop improvement. Theoretical and Applied Genetics 102: 1292-1298.
Upadhyaya HD. 2005. Variability of drought resistance related traits in the mini core collection of peanut. Crop Science 45: 1432-1440.
Vadez V, Krishnamurthy L, Serraj R, Gaur PM, Upadhyaya HD, Hoisington DA, Varshney RK,Turner NC and Siddique KHM. 2007. Large variation in salinity tolerance in chickpea is explained by differences in sensitivity at the reproductive stage. Field Crops Research 104: 123-129.
Wright GC, Nageswara Rao RC and Farquhar GD. 1994. Water use efficiency and carbon isotope discrimination under water deficit conditions. Crop Science 34: 92-97.
Xu W, Rosenow DT and Nguyen HT. 2008. Stay green trait in grain sorghum: relationship between visual rating and leaf chlorophyll concentration. Plant Breeding 119: 365 – 367.
Yadava UL. 1986. A rapid and nondestructive method to determine chlorophyll in interact leaves. Horticultural Science 21: 1449- 1450.
Zahran HH. 1999. Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiology and Molecular Biology Review 63: 968-89.
Zaidi PH, Mamata Yadav, Singh DK and Singh RP. 2008. Relationship between drought and excess moisture tolerance in tropical maize (Zea mays L.). Australian Jornal of Crop Science 1: 78-96
