Rhizobial strains of the Indian Thar Desert and their role in enhanced mungbean (Vigna radiata L.) cultivation at the Gangetic Plains
DOI:
https://doi.org/10.53550/jfl.v38.i2.270Keywords:
Mungbean, PGPR, Phylogenetic tree, Root nodules, Rhizobia, Rhizospheric soil, Thar DesertAbstract
A field survey was carried out to isolate effective strains of Rhizobium from the Indian Thar Desert and applied them to mungbean to assess their impact on enhancing production and improving soil fertility. Based on morphological, biochemical, and physiological characterization, 10 out of 26 isolates were identified as Rhizobium and exhibited positive results for nitrogen fixation, siderophore production, phosphate solubilization, and antagonistic activity against Aspergillus niger green mold. A Rhizobial consortium was formed using these potential PGPR strains and applied as a co-inoculant to mungbean to assess its impact on plant growth under field conditions. The Rhizobial consortium + 50% NPK was found significant than the Rhizobial consortium alone. Whereas Rhizobial consortium treatment without NPK was also impactful in comparison with uninoculated control. This study aimed to evaluate the potential of Rhizobial consortia of Indian Thar and their efficacy in Gangetic plains.
References
Ahemad M and Kibret M. 2014. Mechanisms and applications of plant growth-promoting rhizobacteria: Current perspective. Journal of King Saud University Science 26(1): 1-20.
Anandaraj B and Delapierre ALR. 2010. Studies onnfluence of bioinoculants (Pseudomonas flourescens, Rhizobium, and Bacillus megaterium) in green gram. Journal of Bioscience and Technology 1(2): 95-99.
Arnon DI. 1949. Copper enzymes in isolated chloroplasts: polyphenol oxidases in Beta vulgaris. Plant Physiology 24: 1–14
Arora NK, Verma M and Mishra J. 2017. Rhizobial bioformulation: Past, present, and future. In: S Mehnaz (Ed), Rhizotrophs: Plant growth promotion to bioremediation. Springer Nature Singapore. Pp 69-99.
Ashry NM, Bothaina A, Alaidaroos SA, Mohamed, Omnia AM, Mohamed T, Saadony EL and Ahmed E. 2022. Utilization of drought-tolerant bacterial strains isolated from harsh soils as a plant growth-promoting rhizobacteria (PGPR). Saudi Journal of Biological Sciences 29(3): 1760-1769.
Baldani, JL, CarusoVL, Baldani LD, Silvia RG and Dobereiner J. 1980. Recent advances in BNF with nonlegume plants. Soil Biology and Biochemistry 29(6): 911-922.
Barea JM, Pozo MJ, Azcón R and Azcón-Aguilar C. 2005. Microbial co-operation in the rhizosphere. Journal of Experimental Botany 56(417): 1761-1778.
Barry AL, Bernsohn KL, Adams AP and Thrupp LD. 1970. Improved 18-hour methyl red test. Applied Microbiology 20(6): 866-70.
Bisby F. 1994. Phytochemical Dictionary of the Leguminosae, 1st Ed. Chapman and Hall/CRC. Caamano MS, Gerding M, Vargas M, Elizondo EM, Oyarzua P and Campos J. 2018. Lentil (Lens culinaris L.) growth promoting rhizobacteria and their effect on nodulation in coinoculation with rhizobia. Archives of Agronomy and Soil Science 64(2): 244–256.
Canbolat MY, Bilen S, Cakmakc R, Sahin F and Aydyn A. 2006. Effect of plant growth-promoting bacteria and soil compaction on barley seedling growth, nutrient uptake, soil properties and rhizosphere microflora. Biology and Fertility of Soils 42: 350-357.
Das T, Mahapatra S and Bhushan BT. 2024. Molecular identification and characterizations of rhizobacterial isolates collected from lentil rhizosphere of Indogangetic plains. Brazilian Journal of Microbiology 55(2): 1897–1911.
Deshwal VK and Chaubey A. 2014. Isolation and Characterization of Rhizobium leguminosarum from Root Nodule of Pisum sativum L. Journal of Academia and Industrial Research 2(2): 464-467.
Eardly BD, Nour SM, van Berkum P and Selander RK. 2005. Rhizobial 16S rRNA and dnaK genes: mosaicism and the uncertain phylogenetic placement of Rhizobiumg alegae. Applied and Environmental Microbiology Journal 71(3): 1328-1335.
El-Nahrawy and Omara. 2017. Effectiveness of Coinoculation with Pseudomonas koreensis and Rhizobia on Growth, Nodulation and Yield of Common Bean (Phaseolus vulgaris L.). Microbiology Research Journal International 21(6): 1-15.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. International Journal of Organic Evolution 39(4): 783–791.
Gopalakrishnan S, Srinivas V, Alekhya G, Prakash B, Kudapa H and Varshney RK. 2015. Evaluation of Streptomyces sp. obtained from herbal vermicompost for broad spectrum of plant growth-promoting activities in chickpea. Journal of Organic Agriculture 5(2): 123-133.
Hemanta KM and Rajesh G. 2024. Screening for droughttolerant mungbean root nodule bacteria with multiple plant growth promoting traits in Aridisol. Applied Soil Ecology 201: 0929-1393.
Hosseini A, Maleki A, Fasihi K and Naseri R. 2014.The Coapplication of Plant Growth Promoting Rhizobacteria and Inoculation with Rhizobium Bacteria on Grain Yield and Its Components of Mungbean (Vigna radiata L.) in Ilam Province, Iran. World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 8(7): 776-781.
International Legume Database & Information Service (ILDIS) 2006.
Khanna V and Sharma P. 2011. Potential for enhancing lentil (Lens culinaris) productivity by co-inoculation with PSB, plant growth-promoting rhizobacteria, and Rhizobium. The Indian Journal of Agricultural Sciences 81(10): 223-231.
Kimura MA. 1980. Simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120.
Korir H, Mungai NW, Thuita M, Hamba Y and Masso C. 2017. Co-inoculation Effect of Rhizobia and Plant Growth Promoting Rhizobacteria on Common Bean Growth in a Low Phosphorus Soil. Frontiers in Plant Science 7(8): 141-148.
Koser SA. 1923. Utilization of the Salts of Organic Acids by the Colon-Aerogenes Group. Journal of Bacteriology 8(5): 493-520.
Laranjo M, Alexandre A and Oliveira S. 2014. Legume growth-promoting rhizobia: An overview on the Mesorhizobium genus, Microbiological Research 169(1):2-17.
Lupwayi NZ, Clayton GW, Hanson KG, Rice WA and Biederbeck VO. 2004. Endophytic rhizobia in barley, wheat, and canola roots. Canadian Journal of Plant Science 84(1): 37-45.
MacFaddin JF. 2000. Biochemical tests for identification of medical bacteria, 3rd Ed. Lippincott Williams & Wilkins, Philadelphia, PA.
MacFaddin JF. 1976. Biochemical tests for Identification of Medical bacteria. Williams and Wilkins Co, Baltimore, USA.
Mayz J, Manzi L and Larez A. 2013. Isolation, characterization and identification of hydrocarbonoclastic Pseudomonas spp. Inhabiting the rhizosphere of Crotalaria micans. Journal of Experimental Biology 3(5): 313-321.
Megu M, Paul A and Chitta RD. 2024. Isolation and screening of stress-tolerant and plant growth promoting root nodulatingrhizobial bacteria from some wild legumes of Nagaland, India. South African Journal of Botany 168(1): 260-269.
Nohwar N, Khandare RV and Desai NS. 2019. Isolation and characterization of salinity tolerant nitrogen fixing bacteria from Sesbania sesban root nodules. Biocatalysis and Agricultural Biotechnology 21(1): 140-152.
Pikovskaya RI. 1948. Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiology 17(1): 362–370.
Robinson GHJ and Balk J. 2019. Domoney C. Improving pulse crops as a source of protein, starch, and micronutrients. Nutrition Bulletin 44(3): 202-215.
Schwyn B and Neilands JB. 1987. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160(1): 47-56.
Sehrawat N, Bhat KV, Kaga A, Tomooka N, Yadav M and Jaiswal PK. 2014. Development of new genespecific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek). Spanish Journal of Agricultural Research 12(3): 732-741.
Sessitsch A, Howieson JG, Perret X and Martine-Romero. 2002. Advance in Rhizobium Research. Current Research in Biological Sciences 21(4): 323-378.
Shaharoona B, Arshad M and Zahir ZA. 2006. Effect of plant growth promoting rhizobacteria containing ACC-deaminase on maize (Zea mays L.) growth under axenic conditions and on nodulation in mungbean (Vigna radiata L.). Letters in Applied Microbiology 42(2): 155-169.
Shahzad F, Muhammad S, Abbas F, Babar S, Mohammad T and Ahmad Z. 2012. Isolation and biochemical characterization of Rhizobium meliloti from root nodules of Alfalfa (Medico sativa). Journal of Animal and Plant Sciences 22(1): 522-524.
Sharma NK, Panwar PK and Kumawat N. 2017. Evaluation of mungbean varieties and production technologies at farmer’s fields in western Rajasthan. Annals of Arid Zone 56(2): 43-45.
Singh B, Kaur R and Singh K. 2008. Characterization of Rhizobium strain isolated from the roots of Trigonella foenum graecum (fenugreek). African Journal of Biotechnology 7(1): 3671-3676.
Skidmore AM and Dickinson CH. 1976. Colony interactions and hyphal interference between Septoria nodorum and phylloplane fungi. Transactions of the British Mycological Society 66(1): 57-64.
Srivastava P, Pal DK, Manini A, Wani SP, Kanwar L and Sahrawat. 2015. Soils of the Indo-Gangetic Plains: a pedogenic response to landscape stability, climatic variability and anthropogenic activity during the Holocene. Earth-Science Reviews 140: 54-71.
Tamura K, Glen S and Sudhir K. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38(7): 3022–3027.
Vedder EB. 1915. Starch Agar, A Useful Culture Medium, The Journal of Infectious Diseases 16(3): 385-388.
Verma M, Verma S and Arora NK. 2017. Application of Rhizobium-Pseudomonas consortia for enhanced production of mungbean in sustainable manner. International Journal of Science, Technology and Society 3(2): 54-61.
Vidhyasekaran P and Muthamilan M. 1999. Evaluation of a powder formulation of Pseudomonas fluorescens pf1 for control of rice sheath blight. Biocontrol Science and Technology 9(2): 67-74.
Wei W, Guan D, Ma M, Jiang X, Fan F, Meng F, Li L, Zhao B, Zhao Y, Cao F, Chen H and Li J. 2023 Longterm fertilization coupled with Rhizobium inoculation promotes soybean yield and alters soil bacterial community composition. Frontiers in Microbiology 14(1): 61-76.
Weller DM and Cook RJ. 1983. Suppression of takeall of wheat by seed treatments with fluorescent pseudomonads. Phytopathology 73(4): 463-469.
Yadav DL, Jaisani P and Pandey RN. 2014. Identification of sources of resistance in Mungbean genotypes and influence of fungicidal application to powdery mildew epidemics. International Journal of Current Microbiology and Applied Sciences 3(2): 513-519.




