Bioassay for bruchid resistance in cowpea
DOI:
https://doi.org/10.53550/jfl.v22i1.1841Keywords:
Bioassay, Bruchid, Callosobruchus chinensis L, Resistance, Vigna unguiculata (L.) WalpAbstract
Thirty genotypes of cowpea were screened for bruchid resistance under laboratory condition and they were also assayed for Amylase inhibitor activity. Observations were recorded for total number of eggs laid, per cent adult emergence, extent of seed damage (total holes/twenty seeds, number of holes per seed, type of holes in each seed) and per cent weight loss of seeds. The genotypes have shown differential level of resistance. Amylase inhibitor activity among the genotypes had considerable variability, majority of genotypes exhibited positive correlation between amylase inhibitor activity and bruchid resistance. However, presence of high activity of amylase inhibitor among some susceptible genotypes further includes possibilities of involvement of other factors in addition to amylase inhibitor against the bruchid.
References
Adjadi O, Singh BB and Singh SR. 1985. Inheritance of bruchid resistance in cowpea. Crop Science 25(5): 740-742.
Bressani R. 1995. Nutritive value of cowpca. In: SR Singh and KO Rachie (Eds.). Cowpea Research, Production and Utilization. John Wiley and Sons. Uhichester, U.K. Pp.353-359.
Das A. Patgiri P and Bidyut SK. 2005. Callosobruchus chinensis L. (Bruchids) cause damage to number of important pulse grain's during storage. Legume Research 28(1): 74-76.
Gatehouse AMR. Gatehouse JA. Dobie P. Kilminster and Boulter D. 1979. Biochemical basis of insect resistance in Vigna unguiculata. Journal of the Science of Food and Agriculture 30: 948-958.
Pandey ID, Singh BB and Kumar D. 2007. Present status and future prospects of cowpea research in India. Journal of Arid legumes 4(1): 26-30.
Piergiovanni AR. Sonnate G. Gatta CD and Perrino P. 1991. Digestive enzyme inhibitors and storage pest resistance in cowpea (Vigna unguiculata) seeds. Euphytica 54:191-194.
Redden RJ and McGuire J. 1983. The genetic evaluation of bruchid resistance in seed of cowpea [Vigna unguiculata, Callosobruchus maculatus, storage pest. Nigeria). Australian Journal of Agricultural Research 34(6): 707-715.
Singh BB. Singh SR and Adjadi O. 1985. Bruchid resistance in cowpea. Crop Science 25(5): 736-739.
Schroeder HE. Gollasch S. Moore A. Tabe LM, Craig S. Hardie DC. Chrispeels MJ. Spencer D and Higgins TJV. 1995. Bean alpha- amylase inhibitor confers resistance to the pea weevil (Bruchus pisorum) in transgenic peas (Pisum sativum L.). Plant Physiology 107(4): 1233-1239.
