RAPD markers for identification of cytoplasmic genic male sterile, maintainer and restorer lines of pigeonpea
DOI:
https://doi.org/10.53550/jfl.v21i4.1901Keywords:
C. cajan, C. scarabaeoides, C. sericeus, CMS system, Marker, Pigeonpea, RAPDAbstract
The cytoplasmic-genetic male sterility (CMS) system is considered to be the feasible approach to develop hybrids in pigeonpea. Identification of CMS lines, their maintainers and putative restorers using molecular markers in the early stage of growth is important and economical in the long duration pigeonpea. Random amplified polymorphic DNA (RAPD) markers were used to identify CMS lines derived from crosses between wild (Cajanus scarabaeoides and C. sericeus) and cultivated (C. cajan) pigeonpea. Of the 108 RAPD primers screened, 80 were found to be polymorphic. A set of RAPD primers were identified that could distinguish the CMS systems of GT 288 A/B and 67 A/B. Moreover, specific primers differentiating the CMS lines (GT 288A/67A), maintainers (GT 288B/67B) and putative restorers (ICP 41 and DPPA 85-7) were identified. The use of these primers in heterosis breeding is discussed.
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