Genetic diversity in mungbean as revealed by SSR and ISSR markers
DOI:
https://doi.org/10.53550/jfl.v21i1.1990Keywords:
ISSR, Mungbean, PCA, SSR, ligna radiata L.Abstract
Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles helps to generate breeding populations with broad genetic base. In order to obtain an overview of the genetic diversity present in mungbean, 30 genotypes were analyzed at the DNA level by simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) markers. Nineteen SSR and 35 ISSR primers were used of which, 10 and 20 primers respectively showed amplification. Of the 10 SSR primers 7 showed polymorphism with 1.6 polymorphic fragments per primer. The SSR primers amplified 1 (VR2,VR3,VR4, MB14 & MB77) to 3 (VR9F and 9R) alleles of 150 bp to 300 bp. The 20 ISSR primers which showed amplification produced 116 bands of which 72 were polymorphic (62%). The number of amplified bands ranged from 2 (ISSR807,809,819,820) to 12 (ISSR842) and the size ranged from 200 bp to 2,700 bp. Primers based on AC motif produced more polymorphism per primer (7.3) in comparison to AG motifs based primers (2). Dendrogram based on SSR and ISSR data grouped the 30 mungbean genotypes into five clusters. Dendrogram indicated clear pattern of clustering of genotype according to the location from which they were collected. The pattern of clustering was also reiterated by the grouping by principal component analysis (PCA). The genotype Madhira mung was represented as separate Operational Taxanomic Unit (OTU) showing low similarity coefficient with the other genotypes studied. Genotypes Mulmarada and Karnataka Local grouped separately, while Samrat formed a separate OTU. The results indicate the usefulness of SSR and ISSR markers in the assessment of genetic variability among the mungbean genotypes.
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