Frequency and spectrum of chlorophyll mutants in mungbean
DOI:
https://doi.org/10.53550/jfl.v20i2.2035Keywords:
Chlorophyll mutants, Mutagens, Mungbean, ligna radiata.Abstract
A comparative study of frequency and spectrum of chlorophyll mutants induced by chemical mutagens (EMS and SA) and physical mutagen (Gamma radiation) in M, generation of two local varieties of mungbean viz., Vaibhav and Kopargaon I was undertaken. The treatments included were four concentrations of EMS and SA viz., 0.01, 0.02, 0.03 and 0.04 M and three doses of gamma radiation (30 KR, 40 KR and 50 KR). The results showed that SA and gamma radiation induced three types of chlorophyll mutation viz., straita, chlorina and xantha while EMS induced straita, chlorina, xantha, albina and variegated mutants. EMS was found to be most efficient in inducing chlorophyll mutation. Among the mutations, chlorina appeared in maximum frequency. The highest frequency of mutation was found in variety Vaibhav. Presence of wide spectrum and high frequency of mutants indicated that Vaibhav was more sensitive to mutagens than Kopargaon 1.
References
Natrajan AT and Upadhyay MD. 1964. Localised chromosome breakage induced cthy methane sulphonate and hydroxylamine in Vicia faha Chromosome 15: 156-169.
Rao SM, Swamy L and Hushim H 1984. Mutagenic effectiveness and efficiency of recurrent and sequential treatments of gamma rays and EMS on Vigna radiata (L.) Wilczek Genetica Agraria 38: 399-413.
Reddy VRK and Gupta PK. 1989. Induced mutations in triticales: Frequeeny and spectrum of chlorophyll mutations. Indian Journal of Genetics and Plant Breeding 49: 183-190.
Sharma R. Singh VP, Srivastava A and Sharma SK. 1995 The influence of copper on the mutagenic efficiency of EMS in mungbean. Journal of Indian Botanical Society 74: 81-83.
Singh VP. Singh M and Lal JP. 2000. Gamma rays and EMS induced genetic variability for quantitative trait in urdbean (ligna mungo Hepper). Indian Journal of Genetics and Plant Breeding 60(1): 89-96.
Stadler LJ. 1930. Some genetic effects of X-rays in plants. Journal of Heredity 21: 3-19.




