Phylogenetic analysis of pulse crops using RAPD markers
DOI:
https://doi.org/10.53550/jfl.v20i1.2067Keywords:
Cluster analysis, Genetic identity, Polymorphism, Pulses, RAPDAbstract
RAPD analysis of 16 varieties representing eight different pulse crops namely, pea, lentil, lathyrus, chickpea, pigeonpea, frenchbean, urdbean and mungbean indicated that RAPD primers can effectively be used to establish genomic relationship among the pulse crops. Out of 40 random decamer primers used in the analysis, 30 resulted in polymorphic amplification products in the range of 4 to 11 with amplicon size ranging from 0.2 to 3.5 kb. Cluster analysis using NTSYS showed distinct genotypic group for each crop i.e., no variety of a particular crop was grouped with varieties of other crops. At the species level, chickpea and lentil were grouped together, whereas, pea and lathyrus were close to each other. The two crops of the genus Vigna, mungbean and urdbean, were grouped together with their varieties forming separate sub-clusters. frenchbean and pigeonpea were grouped in distinct clusters. The present analysis shows that with appropriate primer choice and reaction conditions, RAPD can be a very useful tool for phylogenetic analysis of pulse crops.
References
Abdelnoor RV, Barros EG and Moreira MA. 1995. Determination of genetic diversity within Brazilian soybean germplasm using random amplified polymorphic DNA techniques and comparative analysis with pedigree data. Brazilian Journal of Genetics 18: 265-273
Ajibade SR. Weeden NF and Chile SM. 2000. Inter simple sequence repeat analysis of genetic relationships in the genus ligna. Euphytica 111: 47-55.
Choi HK, Mun JH, Kim DJ, Zhu H. Back JM. Mudge J. Roc B. Ellis. THN, Doyle J. Kiss GB, Young ND and Cook DR. 2004. Estimating genome conservation between crops and model legume species. Proceedings of National Academy of Science (USA) 101: 15389- 15394.
Doyle JJ and Luckow MA. 2003. The rest of the iceberg. Legume diversity and evolution in a phylogenetic context. Plant Physiology 131; 900-910.
Fatokun CA, Menancio-Hautca DI, Danesh D and Young ND. 1992. Evidence for orthologous seed weight genes in cowpea and mungbean based on RFLP mapping. Genetics 132: 841-846.
Jaccard P. 1908. Nouvelle recherches sur La distribution florale. Bulletin de la Societe Vaudoise des Sciences Naturelles 44: 223-270.
Kaga A. Tomooka N. Egawa Y, Hosaka K and Kamijima O. 1996. Species relationships in the subgenus Ceratotropis (genus l'igna) as revealed by RAPD analysis. Euphytica 88: 17-24,
Kass E and Wink M. 1995. Molecular phylogeny of the Papilionoideac (Family Leguminosae): rbcL gene sequences versus chemical taxonomy. Botanica Acta 108: 149-162.
Rohlf FJ. 1998. NTSYS Pc numerical taxonomy and multivariate analysis system version 2.0. Exeter software New York. Santalla M. Power JB and Davey MR. 1998. Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breeding 117: 473-478.
Saravanakumar P, Kaga A. Tomooka N and Vaughan DA. 2004. AFLP and RAPD analyses of intra- and inter-specific variation in some ligna subgenus Ceratotropis (Leguminosae) species. Australian Journal of Botany 52: 417-424.
Selvi R, Muthiah AR, Maheshwaran M and Shanmugasundaram S. 2003. Genetic diversity in the genus ligna based on morphological traits and isozyme markers. SABRAO Journal 35: 103-112.
Souframanien J and Gopalakrishna T. 2004. A comparative analysis of genetic diversity in- black gram genotypes using RAPD and ISSR markers. Theoretical and Applied Genetics 109: 1687-1693.
Wilde J, Waugh JR and Powell W. 1992. Genetic fingerprinting of Theobroma clones using RAPD markers. Theoretical and Applied Genetics 83: 871-877.
Weder Jurgen KP. 2002. Species identification of beans, peas, and other legumes by RAPD-PCR after DNA isolation using membrane columns. Lebensmittel Wissenschaft Technologic 35: 277-283. Welsh J and McClelland. 1994. DNA fingerprinting by arbitrary primed PCR. PCR Methods and Application 4: $59- s65
Williams SJ, Kubelik AR, Livak KJ, Rafaiski A and Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531-6535.
