Identification of genotype specific markers and assessment of genetic relatedness among pigeonpea cultivars using RAPD
DOI:
https://doi.org/10.53550/jfl.v20i1.2069Keywords:
Cajanus cajan, Genetic relationship, RAPDAbstract
Twenty-one polymorphic RAPD primers providing proper amplification pattern were selected for identification of genotype specific markers and assessment of genetic relatedness among pigeonpea cultivars. Among these, 16 were found to be unique producing 40 genotype specific bands in 16 different genotypes. An average of 12.6 bands per primer was obtained with 89.4% polymorphism. Clustering based on Jaccard's similarity coefficient using UPGMA grouped all the cultivars into two major clusters and clustering was supported by high bootstrap values. The arithmetic mean heterozygosity (H), the average heterozygosity for polymorphic markers (H)p and marker index (MI) were calculated and found high, which indicated the correctness of the generated profile and efficacy of RAPD as a marker system. The genotype specific bands developed by the RAPD primers could be used for cultivar identification.
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