Induced variability in chickpea through EMS, SA and gamma radiation
DOI:
https://doi.org/10.53550/jfl.v20i1.2087Keywords:
Chickpea, EMS, Gamma radiation, Heritability, Sodium azide, VariabilityAbstract
In the present investigation, an attempt was made to induce genetic variability in yield contributing traits of two popular chickpea cultivars, Vijay and Vishwas, employing three well known mutagens, sodium azide (SA), ethyl methane sulphonate (EMS) and gamma radiation (GR). Result indicated that all mutagenic treatments were effective in inducing genetic variability in yield contributing traits in both the varieties. The 2 mM concentration of SA promoted plant spread in both the varieties, seed yield/plant in Vijay and 100-seed weight in Vishwas. Higher concentrations of SA and EMS as well as gamma radiation caused reduction in these traits. The extent of effect was dose dependent. Lower concentration of gamma radiation showed higher value for plant height and 100-seed weight in both varieties. Statistical analysis revealed higher phenotypic coefficients of variation (PCV) than genotypic coefficients of variation (GCV) for all the six quantitative traits studied. High heritability coupled with high genetic advance observed for traits such as plant height, number of pods per plant and number of seeds per plant offer wide scope for selection in breeding programmes of chickpea.
References
Barshile JD, Auti SG, Dalve SC and Apparao BJ. 2006. Mutagenic sensitivity studies in chickpea employing SA, EMS and gamma rays. Indian Journal of Pulses Research 19(1): 43-46.
Bhatnagar SK. 1991. Induced variability in kabuli chickpea (Cicer arietinum L.). In: Proceedings of Symposium on Plant Mutation Breeding for Crop Improvement, 18-22 June 1991, IAEA. Vienna, Vol. 1: 455-462.
Burton GW and DeVane EH. 1953. Estimating heritability in tall Fescue (Festuca arundinaceaea) from replicated clonal material. Agronomy Journal 45: 478-481.
Hanson GH, Robinson HF and Comstock RE. 1956. Biometric studies of yield in segregating populations of Korean and Lespedeza. Agronomy Journal 48: 268-272.
Hasib KM and Kole PC. 2004. Cause and effect relationship for yield and its components in scented rice hybrids involving gamma rays induced mutants. Journal of Nuclear Agriculture Biology 33(1): 49-55.
Johnson HW, Robinson HF and Comstock RE. 1955. Estimates of genetic and environmental variability in Soybean. Agronomy Journal 47: 314-318.
Kumar S and Dubey DK. 1996. Variability and correlation studies in grasspea (Lathyrus sativum L.). FABIS Newsletter 38/39: 26-30.
Rao S and Rao D. 1982. Studies on the effect of X-irradiation on Cyamopsis tetragonoloba L. Proceedings Indian National Science Academy 48: 410-415.
Singh AK and Singh KP. 2003. Induced quantitative variation for yield and its components in okra (Abelmoschus esculentus (L.) Moench) Advances in Plant Sciences 16(2): 519-525.
Singh J and Singh S. 2001. Induced mutations in basmati rice (Oryza sativa L.). In Proceedings of Diamond Jubilee Symposium, Indian Society of Genetics and Plant Breeding, 6-9 November, New Delhi. Pp. 212.
Tadele A, Haddad NI, Malhotra R and Samarah N. 2005. Induced polygenic variability in kabuli chickpea (Cicer arietinum L.) lines. Crop Research 29(1): 118-128.




