Mutagenic sensitivity studies in chickpea employing SA, EMS and gamma rays
DOI:
https://doi.org/10.53550/jfl.v19i1.2273Keywords:
Chickpea, Chlorophyll mutation, Cicer arietinum, Mutagen, Mutagenic sensitivityAbstract
Two chickpea cultivars (Vijay and Vishwas) were used for the study of varietal differences in mutagenic sensitivity. The seeds were treated with three concentrations of SA (2, 3 and 4 mM), EMS (8, 12 and 16 mM) and three doses of gamma radiations (400, 500 and 600 Gy). Attempts were made to study mutagenic sensitivity in chickpea through biological parameters such as % germination, seedling injury, pollen sterility and survival at maturity in M, generation and the frequency of chlorophyll mutations in M, generation on the basis of plant population. There was significant decrease in germination and survival at maturity while seedling injury and pollen sterility increased with increased concentration of mutagen. The frequencies of chlorophyll mutations increased with increase in concentration in Vijay. In Vishwas, frequencies of chlorophyll mutations increased with increase in concentration with exception of 3 mM SA and 600 Gy gamma radiation treatments. EMS 16 mM and 500 Gy gamma radiation treatments induced maximum chlorophyll mutation in variety Vijay and Vishwas, respectively. The 2 mM SA concentration was most effective in both the cultivars.
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