Screening for efficient strains of Bradyrhizobium
DOI:
https://doi.org/10.53550/jfl.v19i1.2285Keywords:
Antibiotic resistance, Leghaemoglobin, NitrogenaseAbstract
Among 157 rhizobial isolates obtained from the root nodules of pigeonpea, mungbean, urdbean, cowpea, groundnut and soybean grown in six different places of Assam, only 15 strains viz., A., A., B1, B1s B52 C2, C10 G, G15, G., Gn,, Gn, S,, S, and S, were found to be nodulation positive and identified as Bradyrhizobium. Among the 15 strains, Gn, was found the most efficient strain giving the highest acetylene reduction assay (479.01 mmole g''h') and leghaemoglobin content (0.125 mM). On the other hand, strain C, showed the highest efficiency in antibiotic resistance against nine different antibiotics and wide host range infectivity nodulating cowpea, urdbean, mungbean and pigeonpea.
References
Alexander, M. 1985. Ecological constraints on nitrogen fixation in agricultural ecosystems. Advances in Microbial Ecology 8: 163-180.
Appleby, C.A. 1984. Leghaemoglobin and Rhizobium respiration. Annual Review of Plant Physiology 35: 443-478.
Barua, R. and Borthakur, H.P. 1997. Antibiotic disc assay for selection of competitive strains of Rhizobium. Advances in Plant Science 1: 77-79.
Bergersen, F.J. and Goodchild, D.J. 1973. Cellular location and concentration of leghaemoglobin in soybean root nodules. Australian Journal of Biological Sciences 26: 741-757.
Dadarwal, K.R., Prabha, S., Tauro, P. and Subba Rao, N.S. 1977. Serology and host range infectivity of 'cowpea group' rhizobia. Indian Journal of Experimental Biology 15: 462-465.
Dunigan, E.P., Bollich, P.K., Hutchinson, R.N., Hicks, P.M.. Zaunbrecher, F.C., Scott, S.G. and Mowers, R.P. 1984. Introduction and survival of an inoculant strain of Rhizobium japonicum in soil. Agronomy Journal 76: 463-466.
George, M.L.C. and Robert, F.M. 1992. Competition among Rhizobium leguminosarum bv. phaseoli strains for nodulation of common bean. Canadian Journal of Microbiology 38: 157- 160.
Hardy, R.W.F., Holsten, R.D., Jackson, E.K. and Burns, R.C. 1968. The acetylene-ethylene assay for N, fixation: laboratory and field evaluation. Plant Physiology 43: 1185-1205.
Hernandez-Lucas, I., Segovia, L., Mertinez-Romero, E. and Pucppke, S.G. 1995. Phylogenetic relationships and host range of Rhizobium spp. that nodulate Phaseolus vulgaris L. Applied Environmental Microbiology 61(7): 2775-2779.
Sadasivam, S. and Manickam, A. 1996. Pigments. In: Biochemical Methods (2nd Ed.). New Age International (P) Ltd. Publishers: 190-191.
Scotti, M.R.M.M.L., Sa, N.M.H., Vargas, M.A.T. and Dobereiner. J. 1982. Streptomycin resistance of Rhizobium isolates from Brazilian cerrados, Analis de Academia Brasileira de Ciencias 54: 733-738.
Somasegaran, P. and Hoben, H.J. 1994. Handbook for Rhizobia. Springer-Verlag. New York: 1-438.
Tilak, K.V.B.R., Jauhri, K.S. and Saxena, A.K. 1995. Rhizobium Biofertilizer Technology for Legumes. Division of Microbiology, IARI, New Delhi: 1-32.
