Unraveling heat tolerance in mungbean: insights from morpho-agronomical and physio-biochemical traits

Authors

  • Sarvmittar Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141 004, India. Author
  • Ranjit Kaur Gill Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141 004, India. Author
  • Parul Sharma Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141 004, India. Author
  • Satvir Kaur Grewal Department of Biochemistry, Punjab Agricultural University, Ludhiana 141 004, India. Author
  • Shivani Department of Biochemistry, Punjab Agricultural University, Ludhiana 141 004, India. Author

DOI:

https://doi.org/10.53550/jfl.v39i1.2479

Keywords:

Antioxidants, Chlorophyll, Climate resilience, Heat stress, Mungbean, Osmolytes

Abstract

The present study evaluated 36 mungbean genotypes, including interspecific derivatives and mutants, to identify heat-tolerant types based on morphological and physio-biochemical responses. Results revealed significant variability, with MML 2556, SML 2102, and MML 2558 exhibiting superior tolerance, characterized by higher relative leaf water content (RLWC), proline content, and antioxidant activity, whereas ML 2738 and SML 2341 were highly susceptible. Heat stress markedly reduced reproductive traits such as pollen viability, pod length, and seed yield, while oxidative stress markers (malondialdehyde and hydrogen peroxide) increased. Correlation analysis showed positive associations of seed yield with RLWC, proline, carotenoids, and total chlorophyll under stress. Cluster analysis grouped genotypes into tolerant, moderately tolerant, and susceptible categories. The findings highlight the value of physiological and biochemical screening for breeding heat-resilient mungbean cultivars, crucial for sustaining productivity under rising global temperatures.

References

Abdallah MM, El Sebai TN, Ramadan AA and Safwat HM. 2020. Physiological and biochemical role of proline, trehalose and compost on enhancing salinity tolerance of quinoa plant. Bulletin of the National Research Centre 44: 1-13.

Alexieva V, Sergiev I, Mapelli S and Karanov E. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell and Environment 24: 1337-1344.

Anonymous. 2024. IndiaStat. Available at: https://www.indiastat.com.

Bangar P, Chaudhury A, Tiwari B, Kumar S, Kumari R and Bhat KV. 2019. Morphophysiological and biochemical response of mungbean (Vigna radiata L. Wilczek) varieties at different developmental stages under drought stress. Turkish Journal of Biology 43: 58-69.

Barros JRA, dos Santos TC, Silva EGF, da Silva WO, Guimarães MJM and Angelotti F. 2024. Pollen viability and the photosynthetic and enzymatic responses of cowpea (Vigna unguiculata L. Walp., Fabaceae) in the face of rising air temperature: A problem for food safety. Agronomy 14: 463.

Barrs HD and Weatherley PE. 1962. A re-examination of the relative turgidity techniques for estimating water deficits in leaves. Australian Journal of Biological Sciences 15: 413-428.

Bates LS, Woldren RP and Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39: 205-208.

Batra D, Ghosh S, Yadav A and Kumar Y. 2023. Effect of heat stress on growth and yield in mungbean genotypes. Annals of Biology 39: 370-377.

Bhardwaj R, Lone JK, Pandey R, Mondal N, Dhandapani R, Meena SK, Khan S and Gayacharan. 2023. Insights into morphological and physio-biochemical adaptive responses in mungbean (Vigna radiata L.) under heat stress. Frontiers in Genetics 14: 1206451.

Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.

Chadha ML. 2010. Short duration mungbean: A new success in South Asia. APAARI, Bangkok. Pp. 55.

Chand S, Bhatia A and Sharma S. 2020. Effect of heat stress on relative water content and photosynthetic pigments in mungbean. Journal of Plant Physiology 247: 153-160.

Chikukura L, Bandyopadhyay SK, Pathak H and Chakrabarti B. 2017. Effect of elevated temperature stress on growth, yield and yield attributes of mungbean (Vigna radiata) in semi-arid north-west India. Current Advances in Agricultural Sciences 9: 18-22.

Dubois M, Gilles KA, Hamilton JK, Rebers PA and Fred S. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28: 350-356.

Farooq M, Basra S, Wahid A, Cheema Z, Cheema M and Khaliq A. 2008. Physiological role of exogenously applied glycine betaine to improve drought tolerance in fine grain aromatic rice (Oryza sativa L.). Journal of Agronomy and Crop Science 194: 325-333.

Grieve CM and Grattan S. 1983. Rapid assay for determination of water-soluble quaternary ammonium compounds. Plant and Soil 70: 303-307.

Haque MS, Saha NR, Islam MT, Islam MM, Kwon SJ, Roy SK and Woo SH. 2021. Screening for drought tolerance in wheat genotypes by morphological and SSR markers. Journal of Crop Science and Biotechnology 24: 27-39.

Hasanuzzaman M, Nahar K, Alam MM, Roychowdhury R and Fujita M. 2013. Physiological, biochemical and molecular mechanisms of heat stress tolerance in plants. International Journal of Molecular Sciences 14: 9643-9684.

Heath RL and Packer L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125: 189-198.

Hiscox JD and Israelstam GF. 1979. A method for extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332-1334.

Hussain F, Khan EA, Baloch MS, Ullah A, Khakwani AA and Ullah Q. 2022. Impact of seasonal variability on phenological development and productivity of mungbean (Vigna radiata L. R. Wilczek) in arid climatic condition. Applied Ecology and Environmental Research 20: 2985-2999.

Kaur R, Bains TS, Bindumadhava H and Nayyar H. 2015. Responses of mungbean (Vigna radiata L.) genotypes to heat stress: Effects on reproductive biology, leaf function and yield traits. Scientia Horticulturae 197: 527-541.

Mahajan G, Wenham K and Chauhan BS. 2023. Mungbean (Vigna radiata) growth and yield response in relation to water stress and elevated day/night temperature conditions. Agronomy 13: 2546.

Mansoor S and Naqvi FN. 2013. Effect of heat stress on lipid peroxidation and antioxidant enzymes in mungbean (Vigna radiata L.) seedlings. African Journal of Biotechnology 12: 3196-3203.

Mehandi S, Quatadah S, Mishra SP, Singh I, Praveen N and Dwivedi N. 2019. Mungbean (Vigna radiata L. Wilczek): retrospect and prospects. In: Legume crops characterization and breeding for improved food security. Pp. 49-66.

Morales D, Rodríguez P, Dell’Amico J, Nicolás E, Torrecillas A and Sánchez-Blanco MJ. 2003. High temperature preconditioning and thermal shock imposition affects water relations, gas exchange and root hydraulic conductivity in tomato. Biologia Plantarum 47: 203-208.

Nahar K, Hasanuzzaman M and Fujita M. 2015. Modulation of antioxidant machinery and the methylglyoxal detoxification system in mungbean seedlings confers tolerance to drought stress. Frontiers in Plant Science 6: 591.

Navatre RJ, Wagh RS, Mate PR, Jamdade SS, Rajput SD, Chande KB, Narale SB, Keluskar MH and Sadgar TR. 2024. Effect of increasing temperature on phenological and morphological features of mungbean (Vigna radiata L. Wilczek). Plant Archives 24: 703-708.

Neupane MP, Musalman H and Sah SK. 2023. Influence of sowing date on phenology, biometric and yield of mungbean (Vigna radiata) cultivars in Chitwan, Nepal. International Journal of Agronomy 2023: 8927439.

Pandey V and Shukla A. 2015. Acclimation and tolerance strategies of rice under drought stress. Rice Science 22: 147-161.

Priya M, Bhardwaj A, Jha UC, Hanumantha Rao B, Prasad PV, Sharma KD, Siddique KHM and Nayyar H. 2023. Investigating the influence of elevated temperature on nutritional and yield characteristics of mungbean (Vigna radiata L.) genotypes during seed filling in a controlled environment. Frontiers in Plant Science 14: 1233954.

Shahrajabian MH, Sun W and Cheng Q. 2019. A short review of health benefits and nutritional values of mungbean in sustainable agriculture. Polish Journal of Agronomy 37: 31-36.

Sharma L, Priya M, Bindumadhava H, Nair RM and Nayyar H. 2016. Influence of high-temperature stress on growth, phenology and yield performance of mungbean [Vigna radiata (L.) Wilczek] under managed growth conditions. Scientia Horticulturae 213: 379-391.

Siddiqui MH, Al-Khaishany MY, Al-Qutami MA, Al Whaibi MH, Grover A, Ali HM and Al-Wahibi MS. 2015. Morphological and physiological characterization of different genotypes of faba bean under heat stress. Saudi Journal of Biological Sciences 22: 656-663.

Singh V and Collins M. 2024. Genotypic variability in response to heat stress and post-stress compensatory growth in mungbean plants (Vigna radiata [L.] Wilczek). Crops 4: 270-287.

Sita K, Sehgal A, Kumar J, Kumar S, Singh S, Siddique KH and Nayyar H. 2017. Identification of high temperature tolerant lentil (Lens culinaris Medik.) genotypes through leaf and pollen traits. Frontiers in Plant Science 8: 252939.

Swain T and Hillis WE. 1959. Phenolic constituents of Prunus domestica I - the quantitative analysis of phenolic constituents. Journal of the Science of Food and Agriculture 10: 63-68.

Wahid A and Close TJ. 2007. Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves. Biologia Plantarumia an International Journal 51: 104-109.

Wahid A, Gelani S, Ashraf M and Foolad MR. 2007. Heat tolerance in plants: An overview. Environmental and Experimental Botany 61: 199-223.

Zhao Y, Wang Y, Wang L and Zhang D. 2020. Molecular identification of mungbean accessions (Vigna radiata L.) from Northeast China using capillary electrophoresis with fluorescence-labeled SSR markers. Food and Energy Security 9: e182.

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Published

2026-05-15

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How to Cite

Unraveling heat tolerance in mungbean: insights from morpho-agronomical and physio-biochemical traits. (2026). Journal of Food Legumes, 39(1), 30-38. https://doi.org/10.53550/jfl.v39i1.2479