Characterization of pathogen causing chickpea wilt in wet terrace rice cultivation in Kohima, Nagaland
DOI:
https://doi.org/10.53550/jfl.v39i1.2489Keywords:
Cicer arietinum, Fusarium oxysporum, Kohima, Pathogenicity, Wilting pathotype, Yellowing pathotypeAbstract
The present study was conducted to identify the cause of yellowing and wilting of chickpea plants grown under wet terrace rice cultivation (WTRC) fallow in Kohima, Nagaland. Pathogen isolated, from wilt-infected chickpea plants showed fluffy-cottony, thread-like mycelium with pinkish pigmentation at the bottom of the potato dextrose agar plate with microconidia of 5.27×3.10μm, macroconidia of 12.02×2.95μm, and chlamydospores of a diameter of 5.35μm. The isolate was found to be highly pathogenic, showing 94% pathogenicity on four chickpea cultivars reported to be resistant to Fusarium wilt. The original isolate from collected field samples and the re-isolate from the pathogenicity test were confirmed to be Fusarium oxysporum through molecular identification and phylogenetic analysis. This is the first report of F. oxysporum causing chickpea wilt under WTRC in Kohima. The F. oxysporum strain isolated from this study can be used in future breeding programmes to develop resistant chickpea cultivars.
References
Aneja KF. 2003. Experiments in microbiology, plant pathology and biotechnology, 4th Ed. New Age International Publishers, New Delhi. Pp. 30-105.
Booth C. 1971. The genus Fusarium Kew. Commonwealth Mycological Institute, Kew (Surrey), England. Pp 137. Crous PW, Lombard L, Sandoval-Denis M, Seifert KA, Schroers HJ and Chaverri P. 2021. Fusarium more than a node or a foot-shaped basal cell. Studies in Mycologies 98: 100-116.
Daniel A, Gomez-Chavarria, Alvaro L. Rua-Giraldo and Juan FA. 2024. An evolutionary view of the Fusarium core genome. BMC Genomics 25: 304
Dubey SC, Singh SR and Singh B. 2010. Morphological and pathogenic variability of Indian isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Archives of Phytopathology and Plant Protection 43(2): 174-190.
Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791. Geiser DM, Al-Hatmi AMS, Aoki T, Arie T, Balmas V and Barnes I. 2021. Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani species complex. Phytopathology 111(7): 1064-1079.
Golakiya BD, Bhimani MD and Akbani LF. 2018. Characterization of Indian isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt. International Journal of Current Microbiology and Applied Science 7(3): 1152-1162.
Gurjar GS, Barve M, Giri A and Gupta V. 2009. Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene-specific, ITS and random markers. Mycologia 101(4): 484-495.
Haware MP and Nene YL. 1982. Races of Fusarium oxysporum f. sp. ciceri. Plant Disease 66(9): 809-810. Han SL, Wang MM, Ma ZY, Raza M, Zhao P and Liang
JM. 2023. Fusarium diversity associated with diseased cereals in China, with an updated phylogenomic assessment of the genus. Studies in Mycology 104(1): 87-148.
Jiménez-Gasco MM, Pérez-Artés E and Jiménez-Díaz RM. 2001. Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). European Journal of Plant Pathology 107: 237-248.
Jiménez-Gasco MM and Jiménez-Díaz RM. 2003. Development of a specific polymerase chain reaction based assay for the identification of Fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1A, 5, and 6. Phytopathology 93(2): 200-209.
Jiménez-Díaz RM, Castillo P, Jiménez-Gasco M, Landa BB and Navas-Cortes JA. 2015. Fusarium wilt of chickpea: Biology, ecology and management. Crop Protection 73: 16-27.
Kumar S, Stecher G, Suleski M, Sanderford M, Sharma S and Tamura K. 2024. Molecular evolutionary genetic analysis version 12 for adaptive and green computing. Molecular Biology and Evolution 41: 1-9.
Leslie JF and Summerell BA. 2006. The Fusarium laboratory manual. Blackwell Publishing, Ames, Iowa, USA. Pp 388.
Leisso R, Miller Z, Jacobsen B and Burrows M. 2011. Pathogenicity of Fusarium spp. to chickpea seed and seedlings (Cicer arietinum L.). Canadian Journal of Plant Pathology 33(3): 400-409.
Lishma NP, Cherian KA and Mathew D. 2020. Simple and rapid method for extraction of good quality fungal DNA from Fusarium wilt infected banana and molecular characterization of the pathogen. International Journal of Current Microbiology and Applied Sciences 9(6): 2587-2593.
Maitlo SA, Syed RN, Khanzada MA, Rajput AQ, Rajput NA and Lodhi AM. 2014. Host-response of chickpea cultivars to Fusarium oxysporum f. sp. ciceris. Pakistan Journal of Phytopathology 26(2): 193-199.
Moller EM, Bahnweg G, Sandermann H and Geiger HH. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Research 20(22): 6115-6116.
Nene YL, Sheila VK and Sharma SB. 1981. A World list of chickpea and pigeon pea pathogens, 5thEd. International Crops Research Institute for the Semi Arid Tropics 1(1): 27.
Rangaswamy G and Mahadevan A. 1999. Diseases of crop plants in India, 4th Ed. Prentice Hall of India Pvt. Ltd., New Delhi. Pp. 607.
Saitou N and Nei M. 1987. The neighbor-joining method. A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425.
Sankar PM, Shreedevasena S, Karthiba L, Raju AP, Vanitha S, Kamalakannan A and Jeyakumar P. 2024. Ecology, biology and management of Fusarium wilt in chickpea (Cicer arietinum L.): A review. Agricultural Reviews 45(4): 681-686.
SAS Institute Inc. 2023.SAS/STAT®15.3 User’s guide. Cary, NC: SAS Institute.
Sharma KD, Chen W and Muehlbauer FJ. 2005. Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp.ciceris. Plant Disease 89(4): 385-390.
Singh RK, Abul Hasan and Chaudhary RG. 2010. Variability in Fusarium oxysporum f. sp. ciceri causing vascular wilt in chickpea. Archives of Phytopathology and Plant Protection 43(10): 987-995.
Srivastava AK, Dixit GP, Singh NP, Saxena DR, Saabale PR and Raghuvanshi KS. 2021. Evaluation of chickpea (Cicer arietinum L.) accessions for Fusarium wilt resistance. Journal of Environmental Biology 42: 82-89.
Srinivas B, Basha SA, Sagar BV, Kumar CVS and Reddy GK. 2024. Isolation, purification and pathogenicity assessment of Fusarium oxysporum Schl. f. sp. ciceris inciting wilt disease in chickpea. International Journal of Economic Plants 11(2): 153-159.
Sruthy M, Mane SS, Ingle ST, Jadhav PV and Marawar MW. 2024. Race identification and differentiation of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. International Journal of Advanced Biochemistry Research 8(1): 122-128.
Tamura K, Nei M and Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101: 11030-11035.
Trapero-Casas A and Jiménez-Díaz RM. 1985. Fungal wilt and root rot diseases of chickpea in southern Spain. Phytopathology 75(10): 1146-1151.
Venkataramanamma K, Reddy BVB, Jayalakshmi RS, Jayalakshmi V, Prasad KVH and Naidu GM. 2021. Cultural, morphological and molecular characterization of Fusarium oxysporum f. sp. ciceris inciting wilt of chickpea. Legume Research 44(8): 977-
Younesi H, Darvishnia M, Bazgir E and Chehri K. 2021. Morphological, molecular and pathogenic characterization of Fusarium spp. associated with chickpea wilt in Western Iran. Journal of Plant Protection Research 61(4): 402-413.
