Investigating the in vitro regeneration potential of mungbean cultivar Samrat

Abstract

In vitro regeneration of mungbean, being genotype dependent, needs to be standardized with release of new cultivars to be able to use them for further genetic improvement, especially in the regime of genome editing. Samrat is a mungbean cultivar that has reigned mungbean production in past years and is a stable resistant source against Yellow mosaic disease (YMD). The regeneration ability this cultivar, using double cotyledonary node (DCN) and embryonic axis (EA) explants was investigated in Murashige Skoog (MS) medium supplemented with different concentrations of BAP (6-Benzyl Amino Purine) for varied durations. While both the explants responded to in vitro regeneration, number of shoots regenerated was higher with EA (6.45 ± 0.37) than with DCN (5.24 ± 0.37). Sub-culturing thrice on BAP 1.0 mg L-1 supplemented media followed by two subcultures on basal media was optimal for multiple shoot regeneration. Rhizogenesis was obtained on basal media devoid of any phytohormones in EA explants and in 1.0 mg L-1 IAA for DCN explants. The in vitro regenerated plantlets were successfully hardened in a mixture of soil, sand and soilrite (1:1:1) with 72-80% plantlet survival with successful flowering and viable seed setting on maturity. The study revealed that both explants DCN and EA were equally potential explants for in vitro regeneration in Samrat with 1.0 mg L-1 BAP for 30 days.