Prevalence and characterization of Xanthomonas axonopodis pv. cyamopsidis causing bacterial blight of clusterbean in the semi-arid region of North Gujarat

Authors

  • SH Joshi Department of Plant Pathology, Navsari Agricultural University, Navsari- 396450 Author
  • J Purohit Department of Plant Pathology, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506. Author
  • Anirudha Chattopadhyay Pulses Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar -385 506 Author
  • Bhavesh M Joshi Department of Microbiology, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar -385 506. Author

DOI:

https://doi.org/10.59797/jfl.v37.i2.191

Keywords:

Bacterial blight, Cluster bean, Biochemical characterization, Disease survey, Xanthomonas axonopodis pv. cyamopsidis.

Abstract

The bacterial blight caused by Xanthomonas axonopodis pv. cyamopsidis is the most widespread and destructive disease of clusterbean. The present study aimed to survey the major clusterbean growing areas, expanding in five districts i.e., Banaskantha, Patan, Sabarkantha, Mehsana and Aravali of North Gujarat. The study found that bacterial blight disease is prevalent in Idar, Ambaji, Danta, Sanali, Khedbramha, Shamalaji, Patan, Bhiloda, Visnagar, Deesa and Amirgadh. The highest mean disease incidence (28.44%) was recorded in Idar, Sabarkantha. Based on the gram straining it was observed that the test bacterium was gram-negative and rod-shaped. Further, the biochemical tests, such as starch hydrolysis, gelatin liquefaction, hydrogen sulphide production, catalase, and acid production from different sugars viz., sucrose, maltose, and dextrose showed a positive reaction, except, acid production from mannitol was negative. Based on the morphological, biochemical, and pathogenic characteristics, the pathogen was identified as Xanthomonas axonopodis pv. cyamopsidis.

References

Bagri RK, Goyal SK, Singh J and Shekhawat SS. 2019. Management of bacterial leaf blight disease of cluster bean in semi-arid tropic of Rajasthan. Plant Disease Research 34(1): 58-60.

Bhatt RK, Jukanti AK and Roy MM. 2017. Cluster bean [Cyamopsis tetragonoloba (L.) Taub.] an important industrial arid legume: A review. Legume Research 40(2): 207-214.

Chakravarth CN, Krishnapp M and Thippeswamv B. 2005. Isolation and characterization of Xanthomonas axonopodis pv. cyamopsidis in cluster bean (Cyamopsis tetragonoloba (L.) Taub). Asian Journal Microbiology Biotechnology and Environment Science 7(2): 197-200.

Choudhary RS, Yadav RS and Amin A. 2014. Productivity and economics of cluster bean (Cyamopsis tetragonoloba) as influenced by phosphorus fertilization and biofertilizers in western Rajasthan. Annals Agricultural Research 35: 62-64.

Dhaka A, Singh H and Shailza 2019. Growth and instability of cluster bean in Bhilwara district of Rajasthan. Agricultural Science Digest-A Research Journal 39(3): 194-195.

Dye DW. 1962. The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. New Zealand Journal of Science 5(4): 393-416.

Isameldin OA, Yousif Abbo AS, Hamad MAA, Eltahir MES and Elhassan SM. 2018. Occurrence, etiological and ecological aspects of bacterial blight of guar in Sudan. Journal Agriculture Environment Science 18(6): 358-362.

Jadhav RR, More AS, Apet KT and Dandnaik BP. 2018. Cultural and bio-chemical characterization of Xanthomonas axonopodis pv. citri causing citrus canker. International Journal of Current Microbiology and Applied Sciences 6: 1682-1687.

Jonit NQ, Low YC and Tan GH. 2016. Xanthomonas oryzae pv. oryzae, biochemical tests, rice (Oryza sativa), bacterial leaf blight (BLB) disease. Journal of Applied and Environmental Microbiology 4(3): 63-69.

Kumar D and Rodge AB. 2012. Status, scope and strategies of arid legumes research in India. Journal of Food Legume 25: 255-272.

Kumar J and Doshi A. 2018. Pathogenicity test and convenient inoculation method of Xanthomonas axonopodis pv. vignaeradiatae caused leaf spot of green gram. International Journal of Current Microbiology and Applied Science. 7(4): 152-166.

Lodha S. 2001. Efficacy of antagonistic spray on bacterial blight of cluster bean. Indian Phytopathology 54: 133-134.

Mishra S. 2008. Indian guar gum exports up on industry demand. Reuters. April 28, 2008.

Patel CS, Patel JB, Suthar JV and Patel PM. 2010. Effect of integrated nutrient management on cluster bean [Cyamopsis tetragonoloba (L.) Taub.] seed production cv. Pusa Navbahar. International Journal of Agricultural Science 6(1): 206-208.

Patel MK, Dhande GW and Kulkarni YS. 1953. Bacterial leaf spot of Cyamopsis tetragonoloba (L.) Taub. Current Science 22: 183.

Patil B, Karegowda C, Narayanaswamy H and Wasimfiroz M. 2017c. Biochemical variation among isolates of Xanthomonas oryzae pv. oryzae causing bacterial leaf blight in rice. International Journal of Chemical Studies 5(6): 1265-1268.

Rathore BS. 2006. Management of bacterial leaf spot disease of greengram. J Mycol Pl Pathol 36: 153-156.

Ren Y, Yue YL, Jin GX and Du Q. 2014. First report of bacterial blight of guar caused by Xanthomonas axonopodis pv. cyamopsidis in China. Plant Disease 98(6): 840.

Sain SK and Gour HN. 2009. Efficacy of isolated bacteria in vitro inhibition of Xanthomonas axonopodis pv. cyamopsidis and prevention of bacterial leaf blight of cluster bean in the field. Journal of Biological Control 23(4): 421-425.

Salle AJ. 1961. Fermentation of carbohydrates and related compounds. Laboratory manual on fundamental principles of bacteriology. McGraw Hill Book Company, Inc. New York 5: 94-98.

Sandipan PB, Bhanderi GR, Patel RD and Solanki BG. 2017. Survey and status of different diseases of cotton under South Gujarat region, India. International Journal Current Microbiology and Applied Science 6(9): 1362-1367.

Schaad NW. 1992. Xanthomonas. Laboratory guide for identification of plant pathogenic bacteria. 3rd Edn. International Book Distributing Co., Lucknow. p. 165. Shabarishrai P, Dharmatti PR, Shashidhar TR, Patil RV and Patil BR. 2012. Genetic variability studies in cluster bean [Cyamopsis tetragonoloba (L.) Taub]. Karnataka Journal of Agricultural Sciences 25. p. 108.

Sivaprasad Y, Bhaskara Reddy BV, Sujitha A and Sai Gopal DVR. 2012. First report of Tobacco streak virus infecting Cyamopsis tetragonoloba. Journal of Plant Pathology 94 (S4): 96.

Srivastava DN and Rao YP. 1963. Bacterial blight of guar. Indian Phytopathology 16: 69-73.

Vauterin L, Hoste B, Kersters K and Swings J. 1995.Reclassification of Xanthomonas. International Journal of Systemic Bacteriology 45: 472-489.

Vavilov NI. 1951. Phytogeographic basis of plant breeding, the origin, variation, immunity and breeding of cultivated plants. Chronica Botanica 13: 1-366.

Yousif IO, Abbo AS, Hamad MA, Eltahir ME and Elhassan SM. 2018. Occurrence, Etiological and Ecological Aspects of Bacterial Blight of Guar in Sudan. American-Eurasian Journal Agricultural and EnvironmentalSciences 18(6): 358-362.

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Published

2024-07-29

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How to Cite

Prevalence and characterization of Xanthomonas axonopodis pv. cyamopsidis causing bacterial blight of clusterbean in the semi-arid region of North Gujarat. (2024). Journal of Food Legumes, 37(2), 172-180. https://doi.org/10.59797/jfl.v37.i2.191