Efficacy of biocontrol-agents and plant extracts against Sclerotinia sclerotiorum causing stem rot of chickpea
DOI:
https://doi.org/10.59797/jfl.v37.i2.192Keywords:
Bio-agent, Chickpe, Management, Plant extract, Sclerotinia sclerotiorum.Abstract
The present study was conducted to evaluate biocontrol-agents, including Trichoderma viride, T. harzianum and Pseudomonas fluorescens and plant extracts, Ginger, Garlic, Tulsi, Lemon grass and Neem against Sclerotinia sclerotiorum (ON831560.1) under in vitro condition. All the biocontrol-agents suppressed the mycelial growth of S. sclerotiorum. Maximum suppression (69.29%) was observed with T. harzianum, followed by T. viride, which was on par with each other. The Pseudomonas fluorescens was observed to have minimum suppression (61.51%) but more than the control. Among the plant extracts maximum percent inhibition of the pathogenic fungus was observed in treatment with Neem leaf extract (42.58%), followed by garlic clove extract, ginger rhizome extract, and lemon grass leaf, while the minimum per cent inhibition was observed in Tulsi leaf extract (12.30%).
References
Anonymous. 2021. Agricultural statistics at a glance:Ministry of Agriculture & Farmers Welfare, Department of Agriculture & Farmers Welfare, Directorate of Economics and Statistics, Government of India. Pp. 42-43.
Arif M, Verma V, Priyadarshini A, Satnami L, Mishra A, Ansari M, Chattopadhyay A, Bhutia DD and Sarkar A. 2024. Evaluation of Trichoderma spp. as a plant growth promoter and antagonist of major pulse pathogens. Journal of Food Legumes 36(4): 278-287. https://doi.org/10.59797/jfl.v36.i4.164.
Buhariwalla HK, Jayashree B, Eshwar K and Crouch JH. 2005. Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus. BMC Plant Biology 5:16. https://doi.org/10.1186/1471-2229-5-16.
Gupta RP, Singh SK, Mishra RS and Gupta S. 2012. Efficacy of botanicals against Sclerotium rolfsii causing collar rot of chickpea. Annals of Plant Protection Sciences 20(2): 486-487.
Kewate B, Singh D, Singh VD, Malik NP and Kumar R. 2020. In-vitro effect evaluation of botanicals against Sclerotinia sclerotiorum (Lib.) De Bary, caused stem rot disease in Rapeseed-Mustard. International Journal of Current Microbiology and Applied Sciences 9(8): 3733-3741. https://doi.org/10.20546/ijcmas.2020.908.431.
Khalifa GK, Eljack AE, Mohammed MI, Elamin OM and Mohamed ES. 2013. Yield stability in common bean genotypes (Phaseolus vulgaris L.) in the Sudan. Journal of Plant Breeding Crop Science 5(10): 203–208. https://doi.org/10.5897/JPBCS2013.0405.
Manhas A, Sharma R and Dorjey S. 2022. Evaluation of fungicide and bio-control agents against Sclerotinia stem rot of chickpea (Sclerotinia sclerotiorum). The Pharma Innovation Journal 11(2): 358-365.
Nene YL and Thapliyal PN. 1982. Fungicides in Plant Disease Control. Oxford and IBH publishing company private limited, New Delhi Pp. 163.
Pandey P, Kumar R, and Mishra P. 2011. Integrated approach for the management of Sclerotinia sclerotiorum (Lib.) de Bary, causing stem rot of chickpea. Indian Phytopathology, Springer India, New Delhi 64(1): 37-40.
San SH, Sagar D, Kalia VK and Krishnan V. 2022. Effect of different chickpea genotypes and its biochemical constituents on biological attributes of Helicoverpa armigera (Hubner). Legume Research:An International Journal 45(4): 514-520. https://doi.org/10.18805/LR-4474.
Sharma OP and Kumari M. 2018. Management of dry root rot of chickpea through bio agents and soil amendments. Journal of Food Legumes 31(2): 128-130.
Sheshma MK, Kumhar DR, Kumar D, Varma S. and Devi D. 2022. Occurrence and dispersal of sclerotinia rot of chickpea incited by Sclerotinia sclerotiorum in Rajasthan. The Pharma Innovation Journal 11(2): 1696-1700.
Yadav IJ, Gupta VK, Yadav IJ, Verma M and Yadav SR. 2008. Evaluation of botanicals for in vitro management Sclerotinia sclerotiorum causing white mold of Phaseolous lunatus. International Journal of Plant Sciences 4(1): 169-171.
