Identification and characterization of root nodule associated bacteria from chickpea germplasm lines
DOI:
https://doi.org/10.59797/journaloffoodlegumes.v31i4.406Keywords:
Chickpea, Endophytic bacteria, Nodule, Plant growth promotionAbstract
Present study explored the genetic variability of chickpea minicore lines to understand the diversity of endophytic bacteria. A total of 395 nodule associated bacteria from healthy root nodules of chickpea and 30 bacterial isolates were identified based on 16SrDNA sequence similarity and characterized for plant growth promoting traits. BLAST analysis indicated that 16.67% of tested isolates showed only 68-96% sequence similarity with available dataset at NCBI. In total, 22 bacterial isolates belong to Gram negative Genus via. Enterobacter, Rhizobium, Stenotrophomonas, Pseudomonas, and Burkholderia while 8 are Gram positive (Bacillus, and Brevibacillus). Bacterial isolate No. 329B recorded very low 16S rDNA sequence similarity (69%) to Pseudomonas required further analysis for identifying its appropriate taxonomy. He was detected in isolate 281 while nodAB was detected in isolate 365 and 369 of Burkholderia. All 30 bacterial isolates gave negative results for mineral phosphate solubilization as well as HCN production, while 9 isolates produced siderophore. Two bacterial isolates (251A, 265A) were identified for IAA production. Further research on revealing endophytic bacterial diversity and their plant beneficial traits may lead to development and commercialization of microbial formulations to enhance chickpea yield under rainfed condition.
References
Alavi P, Starcher MR, Zachow C, Müller H and Berg G. 2013. Rootmicrobe systems: the effect and mode of interaction of stress protecting agent (SPA) Stenotrophomonas rhizophila DSM14405T. Front Plant Science 4: 141
Ali S, Charles TC and Glick BR. 2012. Delay of flower senescence by bacterial endophytes expressing 1-aminocyclopropane-1- carboxylate deaminase. Journal Applied Microbiology 113: 1139-1144.
Bai Y, Aoust FD, Smith D and Driscoll B. 2002. Isolation of plant growth-promoting Bacillus strains from soybean root nodules. Can Journal Microbiology 48: 230-238.
Bakker AW and Schippers B. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomanas sp. mediated plant growth stimulation. Soil Biology and Biochemistry 19: 451-457.
Baltruschat H, Fodor J, Harrach BD, Niemczyk E, Barna B, Gullner G, Janeczko A, Kogel KH, Schäfer P, Schwarczinger I, Zuccaro A and Skoczowski A. 2008. Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants. New Physiology 180: 501-510.
Cheng D, Tian Z, Feng L, Xu L and Wang H. 2019. Diversity analysis of the rhizospheric and endophytic bacterial communities of Senecio vulgaris L. (Asteraceae) in an invasive range. Peer Journal 6: 61-62.
Choudhary DK, Kasotia A, Jain S, Vaishnav A, Kumari S, Sharma KP and Varma A. 2016. Bacterial-mediated tolerance and resistance to plants under abiotic and biotic stresses. Journal of Plant Growth Regulator 35: 276-300.
Coutinho BG, Licastro D, Previato LM, Cámara M and Venturi V. 2015. Plant-influenced gene expression in the rice endophyte Burkholderia kururiensis M130. Molecular Plant-Microbe Interact 28: 10-21.
Deora GS and Singhal K. 2010. Isolation, biochemical characterization and preparation of biofertilizers using Rhizobium strains for commercial use. Bioscience Biotech Research Communication 3: 132-136.
Dudeja SS, Giri R, Saini R, Suneja Madan P and Kothe E. 2012. Interaction of endophytic microbes with legumes. Journal of Basic Microbiology 52: 248-260.
Edwards U, Rogall T, Blöcker H, Emde M and Bottger EC. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Research 17: 7843-7853.
FAO. 2018. http://www.fao.org/faostat/en/#data/QC Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791.
Gaur PM, Tripathi S, Gowda CLL, Rao GVR, Sharma HC, Pande S and Sharma M. 2010. Chickpea seed production manual. Patancheru 502 324, Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics. pp 28
Haukka K, Lindstrom K and Young JPW. 1998. Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America. Applied of Environmental Microbiology 64: 419-426.
Kandeler E, Deiglmayr K, Tscherko D, Bru D and Philippot L. 2006. Abundance of narG, nirS, nirK, and nosZ genes of denitrifying bacteria during primary successions of a glacier foreland. Applied Environmental Microbiology 72: 5957-5962.
Kumar S, Stecher G and Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology Environmental 33: 1870-1874.
Müller H, Berg C, Landa BB, Auerbach A, Moissl-Eichinger C and Berg G. 2015. Plant genotype-specific archaeal and bacterial endophytes but similar Bacillus antagonists colonize Mediterranean olive trees. Front Microbiology 6: 138.
Nei M and Kumar S. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Saitou N and Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology Environmental 4: 406-425.
Sandhya V, Ali SKZ, Grover M, Reddy G and Venkateswarlu B. 2009. Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45. Biology of Fertilizers Soils 46: 17-26.
Santoyoa G, Hagelsiebb GM, Mosquedac MCO and Glick BR. 2016. Plant growth-promoting bacterial endophytes. Microbiology Research 183: 92-99.
Schwyn B and Neilands JB. 1987. Universal chemical assay for the detection and determination of siderophores. Annals Biochemistry 160: 47-56.
Senthilkumar M, Singh M, Paulraj S, Solai AP and Jagdish S. 2016. Synergistic effect of Mesorhizobium ciceri and ACC deaminase producing rhizobacteria on growth and yield potential of chickpea. Journal Food Legumes 29: 37-42.
Ueda T, Suga Y, Yahiro N and Matsuguchi T. 1995. Remarkable N2- fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences. Journal Bacteriology 177: 1414-1417.
Walitang DI, Kim CG, Kim K, Kang Y, Kim YK and Sa T. 2018. The influence of host genotype and salt stress on the seed endophytic community of salt-sensitive and salt-tolerant rice cultivars. BMC Plant Biology 18: 51.
Waqas M, Khan AL, Kamran M, Hamayun M, Kang SM, Kim YH and Lee IJ. 2012. Endophytic fungi produce gibberellins and indole acetic acid and promotes host-plant growth during stress. Molecules 17: 10754-10773.
Zazueta NE, Acosta OO, Herrera LM, Vazquez RA, López EL, Zúñiga AG and Dorantes AR. 2013. Effect of inoculation with three phytohormone producers phyto-bacteria with ACC deaminase activity on root length of Lens esculenta seedlings. American Journal of Plant Science 4: 2199-2205.
Zhao LF, Xu YJ and Lai XH. 2018. Antagonistic endophytic bacteria associated with nodules of soybean (Glycine max L.) and plant growth-promoting properties. Braz Journal of Microbiology49: 269-278.
