Investigating the change in pathogenesis related proteins (PR) in virus infected cowpea
DOI:
https://doi.org/10.59797/journaloffoodlegumes.v31i4.416Keywords:
BICMV, Cowpea, Proteins, ReiststanceAbstract
Infection due to any pathogen triggers a series of reaction in plant which alters the levels of various pathogenesis related (PR) proteins governing the degree of resistance of that plant. Amongst the various plant viruses infecting cowpea crop, Blackeye cowpea mosaic virus (BICMV) is one of the important and widespread one responsible for reduction in yield of the crop. Changes in the PR proteins viz. -1, 3 glucanase, Phenylalanine ammonia lyase (PAL), peroxidase and polyphenol oxidase were estimated from leaves of cowpea genotypes inoculated with BICMV. The results indicated that resistant varieties had higher levels of PR proteins than moderately resistant. In susceptible variety decreasing trend in PR proteins over the period was recorded indicating that more induction of PR proteins in cowpea is positively correlated with resistance to BICMV.
References
Anuradha C, Selvarajan R, Vasantha S and Suresha GS. 2015. Biochemical characterization of compatible plant virus interaction: A case study with bunchy top virus host pathosystem. Journal of Plant Pathology 14(4): 212-222.
Arora R, Joshi U, Gupta P and Singh J. 2009. Effect of yellow mosaic virus on pathogenesis related enzymes and chlorophyll content in mothbean. Acta Phytopathology Entomology Hungarica 44(1): 49-60.
Bhite BR, Chavan JK and Kachare DP. 1997. A biochemical marker for resistance to sterility mosaic in pigeonpea. Journal of Mahatma phule Agriculture University 22(3): 340-344.
Campos R, Nonogaki H, Suslow T and Saltveit MS. 2004. Isolation and characterization of a wound inducible phenylalanine ammonia lyase gene from romaine lettuce leaves. Physiologia Plantarum 121(3): 429-438.
Hughes JA and shoyinka SA. 2003. Overview of viruses if legumes other than groundnut in Africa. In : Plant virology in sub-saharn Africa. Hughes, J. A. and J. Odu (Eds.). International Institute of tropical Agriculture, Ibadan, Nigeria. Pp: 553-568.
Kale MC and Choudhary AD. 2001. Induction of phenylalanine ammonia lyase in groundnut cultivars in response to biotic and abiotic stress. Indain Phytopathology 54(3): 288-292.
Katoch R, Mann ADS, Sohal BS and Munshi GD. 2004. Effect of elicitor spray and Erysiphe polygoni inoculation on -1, 3
glucanase activity in pea cultivars resistant and susceptible to powdery mildew. Indian Journal Plant Physiology 9(3): 316-319
Kumar KB and Khan PA. 1982. Peroxidase and polyphenol oxidase in excised ragi (Eleusine corocana cv. PR-202) leaves during senescence. Indain Journal of Experimental Biology 20: 412:416.
Lowry OH, Rosenbrough NJ, farr AL and Randall RJ. 1951. Protein measurement with Folin Phenol reagent. Journal of Biological Chemistry 193(1): 265-275.
Mali VR and Kulthe KS. 1980. A seedborne potyvirus causing mosaic disease of cowpea in India. Plant Disease 64(10): 925-928.
Mali VR and Thottappilly G. 1986. Virus diseases of cowpea in the tropics. Review of Tropical Plant Pathology. Vol. 3 (edited by Raychaudhari, S. P. and Verma, J.P.) New Delhi, India; Today and Tomorrow’s printer and Publishers
Nagy NE, Fossdal CG, Dalen LS, Lonneborg A, Heldal I and Johsen O. 2004. Effect of Rhizoctonia infection and drought on peroxidase and chitinase activity in Norway spruce (Picea abies). Physiologia Plantarum 120(3): 465-473.
Patel H, Kalaria R, Mahatma M and Chauhan DA. 2013. Physiological and biochemical changes induced by Mungbean yellow mosaic virus (MYMV) in mungbean (Vigna radiate L.). Journal Cell tissue Research 1: 34.
Rakshit S, Mishra SK, Dasgupta SK and Sharma B. 2000. Dynamics of â-1, 3 glucanase activity in powdery mildew resistant and susceptible lines of pea. Journal of Plant Biochemistry and Biotechnology 9(2): 95-98.
Singh G, Singh K, Kapoor SS, Verma MM, Bhan LK and Sidhu PS. 1987. Pigeonpea lines resistant to sterility mosaic. Indian Phytopathology 40: 18-21.
Somogyi M. 1952. Notes on sugarcane determination. Journal of Biology and Chemistry 195(1): 19-23.
Xu L, Zhu L, Tu L, Liu L, Yuan D, Jin L, Long, L and Zhang X. 2011. Lignin metabolism has a central role in the resistance of cotton to wilt fungus Verticillium dahlia as revealed RNA-seqdependent transcriptional analysis and biochemistry. Journal of Experimental Botany 62(15): 5607-5621.
