Genetic improvement in horse gram [(Macrotyloma uniflorum (lam) verdec.] (syn. Dolichos biflorus L.) through induced mutations
DOI:
https://doi.org/10.59797/journaloffoodlegumes.v29i3&4.493Keywords:
Ethyl Methane Sulfonate, Gamma radiation, Horse gram, Mutagenesis, Sodium AzideAbstract
Horse gram (Macrotyloma uniflorum (Lam) Verdec.) is highly proteinaceous important food legume crop belongs to family Fabaceae. Conventional breeding methods are not useful in enhancing production of horse gram because of low genetic variability. Due to limited genetic diversity and unavailability of good varieties, it is susceptible to several diseases and pests. Induced mutations for various characters using physical and chemical mutagens haven been widely used for induction of mutations in horse gram. Mutagens such as gamma radiations, ethylmethane sulfonate (EMS) and sodium azide (SA) were employed to induce genetic variability in different horse gram cultivars. Different kind of mutations viz., chlorophyll mutations, plant growth habit, pod mutations (small pod, early and tall), seed mutations, maturity mutations (early and late) and yield characteristics were observed in M2 and M3 generations. However, a limited number of efforts have been directed towards understanding quality attributes and resistance to diseases and pests. Mutagenesis if combined with genomic technology may provide powerful tool for the discovery of novel gene alleles underlying several agronomically important traits in this unexploited crop. The present review article will provide information about the use of mutation breeding in genetic improvement of horse gram.
References
Awate PA and Bolbhat SN. 2015. Effect of Ems on Seed Germination Seedling Height and Plant Survival of Horsegram [Macrotyloma Uniflorum (Lam) Verdc] Cv Rayat-1. Journal of Environmental Science Computer Science and Engineering and Technology 4: 272-276.
Bolbhat SN and Dhumal KN. 2009. Induced macromutations in horsegram [Macrotyloma uniflorum (Lam) Verdc]. Legume Research 32: 278-281.
Bolbhat SN and Dhumal KN. 2012. Effect of mutagens on quantitative characters in M2 and M3 generation of Horsegram [Macrotyloma Uniflorum (Lam) Verdc]. International Journal of Scientific and Research Publications 2: 1-7.
Bolbhat SN, Gawade BB, Bhoge VD, Wadavkar DS, Shendage VS and Dhumal KN. 2012a. Effect of Mutagens on Frequency and Spectrum of Viable Mutations in Horsegram (Macrotyloma
uniflorum (Lam) Verdc). IOSR Journal OF Agriculture and Veternary Sciences 1: 45-55.
Bolbhat SN, Bhoge VD and Dhumal KN. 2012b. Induced mutations in horsegram Macrotyloma uniflorum (LAM) VERDC): Chlorophyll mutations, mutagenic efficiency and effectiveness.
International Journal of Life Sciences and Pharmacognosy Research 2: 159-168.
Bolbhat SN and Kamble SR. 2015. Multilocation trials for stability of seed germination percent in M4, M5 and M6 generations of horse gram (Macrotyloma uniflorum (Lam) Verdec.) mutants.
International Journal of Advance Science and Technical Research 5: 211-215.
Datir SS, Dhumal KN and Pandey RN. 2007. Gamma Radiation and EMS induced variation in seed germination and plant survival in Horsegram (Macrotyloma uniflorum (Lam) Verdec) (Syn
Dolichos biflorus L). Journal of Arid Legumes 4: 15-17.
Dhulgande GS, Dhale DA, Pachkore GL and Satpute RA. 2011 Mutagenic Effectiveness and Efficiency of Gamma Rays and Ethyl Methanesulphonate in Pea (Pisum sativum L). Journal of
Experimental Science 2: 07-08.
Durga KK. 2012. Variability and divergence in horsegram (Dolichos uniflorus). Journal of Arid Land 4: 71-76.
Ganiai FA, Khan A, Tario H, Bhat A and Wani NA. 2005. Cytogenetic effects of methylmethane sulfonate (MMS) on two varieties of chickpea (Cicer arietinum L). Journal of Cytology and Genetics 6: 97-102.
Irfaq M and Nawab K. 2001. Effect of gamma radation on some morphological characteristics of three wheat (Triticum aestivum L) cultivars. Journal of Biological Sciences 1: 935-937.
Kanaka KD. Variability and divergence in horsegram (Dolichos uniflorus ) 2012. Journal of Arid Land 4: 71-76.
Kulkarni RN. 1978. Mutagenic effects of gamma rays and EMS in horsegram. Genetica Agricola 32: 65-71.
Kulkarni GB and Mogle UP. 2013. Effects of mutagen on chlorophyll mutation in horse gram [Macrotyloma uniflorum (Lam) Verdcourt]. Bioscience Discovery 4: 214-219.
Mba C. 2013. Induced mutations unleash the potentials of plant genetic resources for food and agriculture. Agronomy 3: 200-231.
Manigopa-Chakraborty Ghosh J, Singh DN, Virk DS and Prasad SC. 2005. Selection in M2 generation of horsegram (Macrotyloma uniflorum) through participatory plant breeding. Journal of Arid Legumes 2: 1-4.
Marki A and Bianu M. 1970. Gamma rays and EMS induced mutations in flax (Linum usitatissimum). Genetika 6: 24-28.
Micke A. 1984. Mutation breeding of grain legumes. Plant and Soil 82: 337-357.
Mou B. 2011. Mutations in Lettuce improvement International Journal of Plant Genomics 2011: 723518. 10.1155/2011/723518.
Patel NB, Tikka NBS, and Patel JB. 2010a. Effect of gamma rays on different parameters under laboratory and field conditions in M1 generation in horsegram. Journal of Food Legumes 23: 14-20.
Patel NB, Tikka NBS, and Patel JB. 2010b. Genetic diversity among selected genotypes of M4 generation in horsegram. Journal of Food Legumes 23: 110-122.
Prakash BG, and Halaswamy KM. 2006 Chemical mutagenesis and their effectiveness under M2 generation in horsegram (Macrotyloma uniflorum (Lam) Verdc). Journal of Arid Legumes 3: 11-14.
Prakash BG, Channayya P, Hiremath Devarnavdgi SB and Salimath PM. 2010. Assessment of genetic diversity among germplasm lines of horsegram (Macrotyloma uniflorum L) at Bijapur. European Journal of Plant Breeding 1: 414-419.
Prasad MVR. 1972. A comparison of mutagenic effectiveness and efficiency of gamma rays, EMS, NMU and NG. International Journal of Genetics 32: 360- 367.
Reddy PCO, Sairanganayakulu G, Thippeswamy M, Reddy PS, Reddy MK and Sudhakar C. 2008. Identification of stress-induced genes from the drought tolerant semi-arid legume crop horsegram (Macrotyloma uniflorum (Lam.) Verdc.) through analysis of subtracted expressed sequence tags. Plant Science 175: 372– 384.
Reddy RR, Maruthi V and Venkateswarlu B 2010. New variety of horsegram (Macrotyloma uniflorum) ‘CRIDA 18R’ released for south India. Indian Journal of Agricultural Sciences 80:477-481.
Rudraswamy P. 1983. Induced mutagenesis inn horsegram (Macrotyloma uniflorum (Lam) Vedec) using gamma rays and ethyl methane sulfonate. Mysore Journal of Agricultural Science
: 95.
Sen NK and Vidyabhushan RV. 1960. Studies on the Induced Polyploids of Horse Gram. Indian Journal of Genetics and Plant Breeding 20: 212-222.
Salini K, Maruthi V, Maheswari M and Sarkar B. 2014. Genetic improvement of horsegram through mutation breeding Agrotechnology 2: 4.
Sharma V, Sharma TR, Rana JC and Chahota RK. 2015 Analysis of Genetic Diversity and Population Structure in Horsegram (Macrotyloma uniflorum) Using RAPD and ISSR Markers. Agriculture Research 4: 221-230.
Shirsat RK, Mohrir MN, Kare MA and Bhuktar AS. 2010. Induced mutations in horsegram: Mutagenic efficiency and effectiveness. Recent Research on Scientific technology 2: 20-23.
Solanki IS and Sharma B. 1999. Induction and isolation of morphological mutations in different mutagenic damage groups in lentil (Lens culinaries Medik). Indian Journal of Genetics and
Plant Breeding 59: 479-485.
Sudha N, Begum JM, Shambulingappa KG and Babu CK. 1995. Nutrients and some anti-nutrients in horse gram (Macrotyloma uniflorum (Lam) Verdc). Food Nutrition Bulletin 16: 1-5.
Varma VP, Durga KK and Ankaiah R. 2013 Assessment of genetic diversity in Horse gram (Dolichos uniflorus). European Journal of Plant Breeding 4: 1175-1179.
Virk DS, Chakraborty M, Ghosh J and Harris D. 2006. Participatory evaluation of horsegram (Macrotyloma uniflorum) varieties and their on-station responses to on-farm seed priming in eastern. Experimental Agriculture 42: 411–425.
Yadava ND and Vyas NL. 1994. Horse gram. In: Arid legumes, India: Agrobotanical publishers, 64-75 pp.
