Trypsin inhibitor activity in developing and germinating seeds of Dolichos biflorus L.
DOI:
https://doi.org/10.59797/jfl.v27i3.792Keywords:
Developing seed, Dolichos biflorus, Germinating seeds, Trypsin inhibitorAbstract
Screening of 15 Dolichos biflorus L. cultivars for trypsin inhibitor activity revealed maximum inhibitor activity in cultivar "HPK4'. Studies on the effect of seed germination and seed development on trypsin inhibitor activity were carried out in this cultivar. Inhibitor activity was detected at early stages of seed development from 3 days after flowering and it increased with seed maturation. During seed germination, trypsin inhibitor activity decreased in germinating seeds with increase in germination period as compared to dry seeds.
References
Alizadeh H, Leung DWM and Edwards Y. 2012. Changes in trypsin inhibitory activity of developing seed parts and pod of red kidney bean. Agronomy New Zealand 42:111-122
Kumari et al.: Trypsin inhibitor activity in developing and germinating seeds of Dolichos biflorus L.
Godbole SA, Krishna TG and Bhatia CR.1994. Changes in protease inhibitor activity from pigeon pea during seed development and germination. Journal of Scientific Food Agriculture 66: 497-501.
Guillamon E, Mercedes M. Pedrosa A, Carmen B, Carmen C,Marý C S and Mercedes M.2008. The trypsin inhibitors present in seed of different grain legume species and cultivar. Journal of Food Chemistry 107:68-74
Kakati P, Deka SC, Kotoki D and Saikia S. 2010. Effect of traditional methods of processing on the nutrient contents and some ant nutritional factors in newly developed cultivars of black gram and green gram of Assam, India. International Food Research Journal 17: 377-384.
Kansal R, Kuhar K, Gupta, RN, Gupta VK and Koundal KR. 2010.Trypsin inhibitor profile in developing and germinating seeds of mungbean and Chickpea. Progressive Agriculture 10: 226-231.
Kansal R, Gupta RN, Koundal KR, Kuhar K, Gupta VK. 2008. Purification, characterization and evaluation of insecticidal potential of trypsin inhibitor from mungbean (Vigna radiata L. Wilczek) seeds. Acta Physiologiae Plantarum 30: 761-768.
Khattab RY and Arntfield SD. 2009. Nutritional quality of legume seeds as affected by some physical treatment: Antinutritional factors. Journal of Food Science and Technology 42: 1113-1118.
Kute LS, Kadam SS, Salunkhe DK. 2006. Changes in Sugars, Starch and Trypsin Inhibitor Activity in Winged Bean (Psophocarpus tetragonolobus L.DC) during seed development. Journal of Food Science 49: 314-315.
Lowry OH, Rosebrough NJ, Farr AL and Randal RJ. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265-75.
Malomo O, Ogunmoyela O A B and Oluwajoba S O. 2011. Effect of sprouting on trypsin inhibitor of cowpea (Vigna unguiculata). Journal of Toxicology and Environmental Health Sciences 3: 139- 141.
Mehta SL and Simlot MM.1982. An acid stable trypsin-chymotrypsin inhibitor from horse gram (Dolichos biflorus). Journal of Biosciences 4: 295-306.
Mohamed KR, Gibriel AY, Rasmy NMH, Ferial MAS and Esmat AA. 2011. Infleunce of legume processing treatments individually or in combination on their trypsin inhibitor and total phenolic contents. African Journal of Food Science and Technology 2: 036-046.
Mubarak AE. 2005. Nutritional composition and anti nutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Journal of Food Chemistry 89: 489-495.
