Genetic diversity assessment in clusterbean (Cyamopsis tetragonoloba (L.) Taub.) by RAPD markers
DOI:
https://doi.org/10.59797/jfl.v27i2.801Keywords:
C. tetragonoloba, Clusterbean, Genetic diversity, RAPD markersAbstract
Genetic diversity analysis of 12 clusterbean (Cyamopsis tetragonoloba (L.) Taub.) genotypes were carried out using Random Amplified Polymorphic DNA (RAPD) markers. The 19 RAPD primers amplified a total of 212 bands, out of which 151 were polymorphic. The size of amplified DNA fragment varied from 146 to 2995 bp. The polymorphic bands varied from 22.22 percent in OPA-11 to 88.88 percent in OPA-12. Dendrogram based Jaccard’s similarity coefficient grouped the 12 genotypes into four major clusters encompassing five subclusters. The first cluster comprised three subclusters with subcluster A1 contained three genotypes viz; GG-2, HG-75 and HG-365. Subcluster B1 had only one genotype GG-1 while, subcluster C1 contained two genotypes viz; RGC-471 and HVG-2-30. Cluster 2 entailed two subclusters viz; B1and B2 having two genotypes each namely PRT-15 and GAUG-0013 grouped in subcluster B1 and FS-277 and PNB in subcluster B2. The third and fourth cluster contained single genotype GAUG-0522 and GAUG-9404, respectively. The similarity index values ranged from 0.52 to 0.87 indicating the presence of enormous genetic diversity at molecular level. Therefore, RAPD analysis could be used as tool for detecting genetic diversity and can be precisely used for grouping and selection of diverse parents. GG-2, HG-75 and HG-365 (Sub group A1) and GAUG-0522 (Group C) may be utilized for breeding good genotypes with high yield and resistance to bacterial blight in clusterbean.
References
Kyaw A, Azahar BSBN and Tunio SQ. 2012. Fracturing fluid (guar polymer gel) degradation study by using oxidative and enzyme Breaker. Research Journal of Applied Sciences, Engineering and Technology 4:1667-1671.
Murray MG and Thompson WF. 1980. Rapid isolation of high molecular- weight plant DNA. Nucleic Acids Research 8:4321- 4325.
Narayan R. 2012. From food to fracking: Guar gum and international regulation. RegBlog. University of Pennsylvania Law School.
Pathak R, Singh SK and Singh M. 2011. Assessment of genetic diversity in clusterbean using nuclear rDNA and RAPD markers. Journal of Food Legumes 24:180-183.
Pathak R, Singh SK, Singh M and Henry A. 2010. Molecular assessment of genetic diversity in cluster bean (Cyamopsis tetragonoloba) genotypes. Journal of Genetics 89:243-246.
Punia A, Yadav R, Arora P and Chaudhury A. 2009. Molecular and morphophysiological characterization of superior cluster bean (Cymopsis tetragonoloba) varieties. Journal of Crop Science and Biotechnology 12:143-148.
Rajora OP and Rahman MH. 2003. Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadiensis) cultivars. Theoretical and Applied Genetics 106:470-477.
Rohlf FJ. 2000. NTSYSpc: numerical taxonomy and multivariate analysis system, version 2.02. Exeter Software, Setauket, New York.
Russell JR, Fuller JD and Macaulay M. 1997. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics 95:714-722.
Virk PS, Newbury HJ, Jackson MT and Ford-Llyod BV. 1995. The identification of duplicate accessions within a rice germplasm collection using RAPD markers. Theoretical and Applied Genetics 90:1049-1055.
Williams JGK, Kubelik AR, Livak KJ, Rafalaski JA and Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18:6531-6535.
