Management of collar rot of chickpea by Pseudomonas fluorescens and identification of sources of resistance
DOI:
https://doi.org/10.59797/jfl.v28i2.980Keywords:
Cicer arietinum L, Chickpea, Collar rot, Pseudomonas fluorescens Resistance, Vigour indexAbstract
An investigation was carried out at Department of Plant Pathology, JNKVV, Jabalpur to know the antagonistic potential of Pseudomons fluorescens against Sclerotium rolfsii. The result of the In vitro testing revealed that highest growth inhibition zone was obtained on King's B medium (82.2%) followed by Pseudomonas Agar (77.1%) and Potato Dextrose Agar medium (62.3%) after 9 days of incubation as compared to control. Combined application of soil and seed treatment of P. fluorescens was found best for increasing germination percentage i.e. 96.6%, 90%, 100% and 86.6% in JG 62, JG 63, JG 315 and JG 74 respectively as compared to seed and soil treatment alone. The combined application of soil and seed treatment of P. fluorescens effectively helped to increase plant growth, vigour index and to minimize disease incidence. Out of 190 chickpea entries comprising of 115 desi and 75 kabuli types evaluated for resistance against S. rolfsii under net house conditions, 18 entries of the desi ware found resistant, however, 12 entries were moderately resistant. In Kabuli type, 3 entries were found resistant, while 4 entries were graded as moderately resistant. In a field screening of 284 chickpea germplasm accessions against collar rot, 9 were found free from disease and 29 exhibited < 10 per cent mortality due to collar rot.
References
Abrahm Mathew K and Gupta SK. 1998. Biological control of root rot of French bean caused by Rhizoctonia solani. Journal of Mycology and Plant Pathology 28:202-205.
Gardener BBM, Schroeder KL, Kalloger SK, Raaijmakers JM, Thomashow LS and Weller DM. 2000. Genotypic and phenotypic diversity of phID-containing Pseudomonas strains isolated from the rhizosphere of wheat. Applied Environment Microbiology 66: 1939-1946.
Gupta O. 2001. Occurrence of sources of resistance in desi and kabuli chickpea genotypes against collar rot and yield attributing character, presented in International chickpea conference held at IGKVV, Raipur (Abst). p. 64.
Gupta O and Babbar A. 2006. Identification of desi and kabuli chickpea genotypes for multiple disease resistance against soil borne diseases. Indian Journal of Pulses Research 19:129-130.
Hameeda B. Harini G, Rupela OP, Rao JVDKK and Reddy G. 2010. Biological control of chickpea collar rot by co-inoculation of antagonistic bacteria and compatible rhizobia. Indian Journal of Microbiology 50: 419-424.
Mukhopadhyay AN. Shrestha SM and Mukherjee PK. 1992. Biological seed treatment for control of soil borne plant pathogens. FAO Plant Protection Bulletin 40: 221-30.
Pal KK. Tilak KVBR. Saxena AK, Dey R and Singh CS. 2000. Antifungal characteristics of a fluorescent Pseudomonas strain involved in the biological control of Rhizoctonia solani. Microbiological Research 155: 233-242.
Raguchander T, Rajappan K and Samiappan R.1997. Evaluating methods of application of biocontrol agents in the control of Mungbean root rot. Indian Phytopathology 50:229-234.
Sharma SK, Verma BR and Sharma BK. 1999. Biocontrol of Sclerotinia sclerotiorum causing stem rot of chickpea. Indian Phytopathology 52:44-46.
Singh A, Verma R and Shanmugam V. 2006. Extracellular chitinases of fluorescent pseudomonads antifungal to Fusarium oxysporum f.sp. dianthi causing carnation wilt. Current Microbiology 52: 310-316.
Singh SP. Agarwal RK and Bhagawati R. 2012. Screening of chickpea germplasms, date of sowing and integrated management of collar rot caused by Sclerotium rolfsii. Annals of Plant Protection Sciences 20: 397-399.
Usharani S. Christopher DJ and Sujaritha A. 2009. Effect of delivery system of Pseudomonas fluorescens on the rhizosphere survival and management of fusarial wilt of tomato. Journal of Biological Control 23: 195-198.
Validov S, Mavrodi O, Fuente L, Boronin A. Weller D. Thomashow K and Mavrodi D. 2005. Antagonistic activity among 2,4- diacetylphloroglucinol producing fluorescent Psedomonads sp. FEMS Microbiology Letters, 242, 249.
Voisard C. Keel C, Haas D and Defago G. 1989. Cyanide production by Pseudomonas fluorescens helps suppress black root rot of tobacco under genobiotic conditions. EMBO J. B., 351-358.
Weller DM. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annual Review of Phytopathology 26: 379-4.
