Identification of butterfly pea (Clitoria ternatea L.) as an alternate host of mungbean yellow mosaic India virus in India
Keywords:
Begomovirus, Butterfly pea, Bipartite, Clitoria ternatea, Genome, Host, Legumoviruses, Mungbean yellow mosaic India virus, PCR, Yellow mosaic diseaseAbstract
In India, plants from non-cultivated, horticultural and agricultural categories are commonly infected with various species of begomoviruses, typically producing yellow or bright yellow mosaic and curling symptoms on leaves. Butterfly pea (Clitoria ternatea L.), a perennial herb, exhibited yellow mosaic disease (YMD) symptoms and was analyzed for the causal agent. Using a PCRbased approach and sequencing followed by nucleotide similarity search, the complete genome of mungbean yellow mosaic India virus (MYMIV) was identified. Full-length DNA-A (2,746 bp) and DNA-B (2,659 bp) components of MYMIV showed maximum nucleotide identity of 98.98% and 93.40%, respectively, with the known isolates of MYMIV. The common regions (~130 bp) shared 87% nucleotide identity. Both DNA molecules contained conserved iterons, a nonanucleotide and GC-rich inverted repeats with the potential to form a stem-loop structure. Therefore, the virus identified in C. ternatea was considered an isolate of MYMIV, further supported by phylogenetic analyses. This is the first report of a MYMIV isolate with its complete annotated genome, identified in C. ternatea.
References
Akram M, Kamaal N, Pratap A, Kumar D, Muin A, Sabale PR, Aidbhavi R, Sunani SK, Rathore M, Gupta S, Singh NP, Dey N, Dixit GP and Nair RM. 2024a. Exploring distribution and genomic diversity of begomoviruses associated with yellow mosaic disease of legume crops from India, highlighting the dominance of mungbean yellow mosaic India virus. Frontiers in Microbiology 15: 1451986. doi:10.3389/fmicb.2024.1451986
Akram M, Kamaal N, Pratap A, Muin A, Agnihotri AK, Rathore US, Rathore M and Singh NP. 2022. Species diversity, phylogeny and evidence of recombination in begomoviruses associated with yellow mosaic disease of moth bean (Vigna aconitifolia) in South India. Journal of Phytopathology 170: 300–314. doi: 10.1111/jph.13079
Akram M, Kamaal N, Pratap A, Muin A, Ahmad S, Agnihotri AK, Shinde P, Saable P and Singh N. 2020. Molecular characterization of begomoviruses causing yellow mosaic disease in Vigna stipulacea and evidence of recombination. Journal of Food Legumes 33: 232–244.
Akram M, Kumar D and Kamaal N. 2024b. Complete genome sequence of a novel bipartite begomovirus infecting butterfly pea (Clitoria ternatea L.) in India. Archives of Virology 170: 7. doi:10.1007/s00705-024-06203-3
Akram M, Kumar D, Saurav S, Saxena M, Saxena DR, Kamaal N and Dixit GP. 2025. Molecular characterization of yellow mosaic disease causing begomoviruses in pigeonpea (Cajanus cajan L.) from three agro-ecological zones of India. Current Microbiology 82: 95. doi: 10.1007/s00284-025-04080-w
Akram M, Naimuddin, Agnihotri AK, Gupta S and Singh NP. 2015. Characterisation of full genome of Dolichos yellow mosaic virus based on sequence comparison, genetic recombination and phylogenetic relationship. Annals of Applied Biology 167: 354–363. doi: 10.1111/aab.12231
Coutts BA, Kehoe MA, Webster CG, Wylie SJ and Jones RAC. 2011. Indigenous and introduced potyviruses of legumes and Passiflora spp. from Australia: biological properties and comparison of coat protein nucleotide sequences. Archives of Virology 156: 1757–1774. doi: 10.1007/s00705-011-1046-4
Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series No. 41: 95–98.
Ilyas M, Qazi J, Mansoor S and Briddon RW. 2009. Molecular characterisation and infectivity of a “Legumovirus” (genus Begomovirus: family Geminiviridae) infecting the leguminous weed Rhynchosia minima in Pakistan. Virus Research 145: 279–284. doi: 10.1016/j.virusres.2009.07.018
Jamil N and Pa’ee F. 2018. Antimicrobial activity from leaf, flower, stem, and root of Clitoria ternatea–A review., in AIP Conference proceedings, (AIP Publishing). doi: https://doi.org/10.1063/1.5050140
Jeyaraj EJ, Lim YY and Choo WS. 2022. Antioxidant, cytotoxic and antibacterial activities of Clitoria ternatea flower extracts and anthocyanin-rich fraction. Scientific reports 12: 14890. doi: 10.1038/s41598-022-19146-z
Kumar D, Akram M and Kamaal N. 2024. Low-cost water boiling method successfully employed to extract DNA from a single whitefly to detect yellow mosaic disease-causing viruses in PCR. Archives of Phytopathology and Plant Protection 57: 1–17. doi: 10.1080/03235408.2024.2374310
Maneesai P, Iampanichakul M, Chaihongsa N, Poasakate A, Potue P, Rattanakanokchai S, Bunbupha S, Chiangsaen P and Pakdeechote P. 2021. Butterfly pea flower (Clitoria ternatea Linn.) extract ameliorates cardiovascular dysfunction and oxidative stress in nitric oxide-deficient hypertensive rats. Antioxidants 10: 523. doi: 10.3390/antiox10040523
Martin D P, Murrell B, Golden M, Khoosal A and Muhire B.
Akram et al. : Identification of butterfly pea (Clitoria ternatea L.) as an alternate host of 421 mungbean yellow mosaic India virus in India
RDP4: Detection and analysis of recombination patterns in virus genomes. Virus evolution 1: vev003. doi: 10.1093/ve/vev003
Morinaga T, Ikegami M and Miura K. 1990. Physical mapping and molecular cloning of mungbean yellow mosaic virus DNA. Intervirology 31: 50–56. doi: 10.1159/000150135
Morris J. 2009. Characterization of butterfly pea (Clitoria ternatea L.) accessions for morphology, phenology, reproduction and potential nutraceutical, pharmaceutical trait utilization. Genetic resources and crop evolution 56: 421–427. doi: 10.1007/s10722-008-9376-0
Muhire BM, Varsani A and Martin DP. 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PloS one 9: e108277. doi: 10.1371/journal.pone.0108277
Nguyen KNT, Nguyen GKT, Nguyen PQT, Ang KH, Dedon PC and Tam JP. 2016. Immunostimulating and Gram-negative-specific antibacterial cyclotides from the butterfly pea (Clitoria ternatea). The FEBS Journal 283: 2067–2090. doi: 10.1111/febs.13720
Qazi J, Ilyas M, Mansoor S and Briddon RW. 2007. Legume yellow mosaic viruses: genetically isolated begomoviruses. Molecular Plant Pathology 8: 343– 348. doi: 10.1111/j.1364-3703.2007.00402.x
Rojas MR, Hagen C, Lucas WJ and Gilbertson RL. 2005. Exploiting chinks in the plant’s armor: evolution and emergence of geminiviruses. Annual Review of Phytopathology 43: 361–394. doi: 10.1146/annurev.phyto.43.040204.135939
Sultana S, Roy B and Sherpa AR. 2019. Natural occurrence of papaya ringspot virus in Clitoria ternatea in India. Journal of Plant Pathology 101: 183–183. doi: 10.1007/ s42161-018-0128-5
Tamura K, Stecher G and Kumar S. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38: 3022–3027. doi: 10.1093/molbev/msab120
Wei K, Gibbs A and Mackenzie A. 2012. Clitoria yellow mottle virus: a tobamovirus from Northern Australia. Australasian Plant Disease Notes 7: 59–61. doi: 10.1007/s13314-012-0048-8
