Beneficial traits of endophytic bacteria from field pea nodules and plant growth promotion of field pea
DOI:
https://doi.org/10.59797/jfl.v26i3&4.871Keywords:
Beneficial characters, Endophytes, field pea, growth promotion, nitrogen fixation, nodules, Rhizobium, symbiosisAbstract
Endophytic bacteria from nodules of field pea (Pisum sativum L.) being grown in CCS Haryana Agricultural University farm were isolated. A total of 60 endophytic bacteria from surface sterilized nodules of field pea were isolated. Screening for the presence of beneficial traits showed that 63.3% of field pea nodule endophytes were promoting root growth of pea seedlings in water agar root growth promotion assay. A total of 38.3% of endophytic isolates were phosphate solubilizers, 83.3% were ammonia producers and only 32% of isolates were producing organic acid. Root growth assay showed that nodule endophytes PNE17 and PNE26 are better root growth promoters; PNE15, PNE24 are best P solubilizers; PNE5, PNE15 are best ammonia producers and PNE17 and PNE27 are the best organic acid producers. Based on the presence of multiple beneficial traits, selected 41 endophytic nodule isolates were inoculated together with Rhizobium leguminosarum biovar viciae strain PS 43 in field pea under pot culture conditions and showed enhanced plant growth, nodulation and nitrogen fixing parameters in field pea. Highest shoot dry weight and shoot N contents was observed in plants inoculated with PNE17 followed by PNE26, PNE77 and PNE15 along with Rhizobium. Symbiotic ratio (SR) based on shoot N contents varied from 0.70 to 3.10 indicating vide variation in effectivity. Endophytic isolate PNE 15, PNE 22, PNE 26 and PNE 77 showed SR ratio more than 2.0, while isolate PNE 17 was the most effective endophyte with SR more than 3.0.
References
Bai Y, Aoust FD, Smith D and Driscoll B. 2002. Isolation of plant growth-promoting Bacillus strains from soybean root nodules. Canadian Journal of Microbiology 48: 230-238.
Bai Y, Zhou X and Smith DL. 2003. Enhanced soybean plant growth resulting from co-inoculation of Bacillus strains with Bradyrhizobium japonicum. Crop Science 43: 1774-1781.
Bremner JM. 1965. Total nitrogen. In: Methods of soil Analysis. (ed. C.A. black). American Society of Agronomy, Madison. 2: 1149–1178.
Bullied J, Buss TJ and Vessey JK. 2002. Bacillus cereus UW85 inoculation effects on growth, nodulation, and N accumulation in grain legumes: field studies. Canadian Journal of Plant Science 82:291–298.
Cappuccino JC and Sherman N. 1992. Microbiology: a laboratory manual, Benjamin/Cummings Publishing Company, New York, pp. 125-179.
Castro-Sowinski S, Herschkovitz Y, Okon Y and Jurkevitch E. 2007. Effects of inoculation with plant growth-promoting rhizobacteria on resident rhizosphere microorganisms. FEMS Microbiology 276(1): 1–11.
Dudeja SS, Giri R, Saini R, Suneja-Madan P and Kothe E. 2012. Interaction of endophytic microbes with legumes, Journal of Basic Microbiology 52: 248–260
Gao Z, Zhuang J, Chen J, Liu X and Tang S. 2004. Population of endophytic bacteria in maize roots and its dynamic analysis. Ying Yong Sheng Tai Xue Bao 15(8): 1344–1348.
Grimes HD and Mount, MS. 1984. Influence of Pseudomonas putida on nodulation of Phaseolus vulgaris. Soil Biology and Biochemistry 16: 27-30.
Hung PQ and Annapurna K. 2004. Isolation and characterization of endophytic bacteria in soybean (Glycine sp.). Omonrice. 12: 92-101.
Khan Z and Doty SL. 2009. Characterization of bacterial endophytes of sweet potato plants. Plant and Soil 322(1-2): 197–207.
Kumar V, Pathak DV, Dudeja SS, Saini R, Giri R, Narula S and Anand RC, 2013. Legume nodule endophytes more diverse than endophytes from roots of legumes or non legumes in soils of Haryana, India. Journal of Microbiology and Biotechnology Research 3 (3):83-92
Lee KD, Bai Y, Smith D, Han HS and Supanjani. 2005. Isolation of plant-growth-promoting endophytic bacteria from bean nodules. Research Journal of Agricultural and Biological Sciences 1(3): 232-236.
Li JH, Wang ET, Chen WF and Chen WX. 2008. Genetic diversity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China. Soil Biology and Biochemistry 40: 238-246.
Nandwani R and Dudeja SS. 2013. Functional diversity of native mesorhizobial genotypes available in Indian soils of Haryana state which nodulates chickpea. Acta Agronomica Hungarica 61(3): 207-217.
Narula S, Anand RC, Dudeja SS, Kumar V and Pathak DV. 2013. Molecular diversity of root and nodule endophytic bacteria from field pea (Pisum sativum L.). Legume Research 36(4): 344-350
Panchal H and Ingle S. 2011. Isolation and characterization of endophytes from the root of medicinal plant Chlorophytum borivilianum (Safed musli). Journal of Advances in Development Research 2 (2): 205–209.
Pikovskaya RE. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya. 17:362-370.
Polonenko DR, Scher FM, Kloepper JW, Singleton CA, Laliberte M and Zaleska I. 1987. Effects of root colonizing bacteria on nodulation of soybean roots by Bradyrhizobium japonicum. Canadian Journal of Microbiology 33: 498-503.
Rajendran G, Sing F, Desai AJ and Archana G. 2008. Enhanced growth and nodulation of pigeon pea by co-inoculation of Bacillus strains with Rhizobium spp. Bioresource Technology 99: 4544-4550.
Saini R, Kumar V, Dudeja SS and Pathak DV 2013a. Beneficial effects of inoculation of endophytic bacterial isolates from roots and nodules in chickpea. Acta Agronomica Hungarica (in press)
Saini R, Dudeja SS, Giri R and Kumar V, 2013b. Isolation, characterization and evaluation of bacterial root and nodule endophytes from chickpea cultivated in Northern India. Journal of Basic Microbiology 53: 1-8
Sambrook J and Russell DW. 2001. Molecular Cloning: A Laboratory Manual, Vol. 1, Cold Spring Harbor, New York.
Selvakumar G, Kundu S, Gupta AD, Shouche YS and Gupta, HS. 2008. Isolation and characterization of non rhizobial plant growth promoting bacteria from nodules of kudzu (Pueraria thunbergiana) and their effect on wheat seedling growth. Current Microbiology 56: 134-139.
Sgroy V, Cassán F, Masciarelli O, Papa MF, Lagares A and Luna V. 2009. Isolation and characterization of endophytic plant growthpromoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopis strombulifera. Applied Microbiology and Biotechnology. 85(2): 371–381.
Stajkovic O, De Meyer S, Milicic B, Willems A and Delic, D. 2009. Isolation and characterization of endophytic non-rhizobial bacteria from root nodules of alfalfa (Medicago sativa L.). Botanica SERBICA. 33(1): 107-114.
Sturz AV, Christie BR, Matheson BG and Nowak J. 1997. Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth. Biology and Fertility of Soils 25: 13-19.
Taurian T, Anzuay MS, Angelini JG, Tonelli ML, Ludueña L, Pena D, Ibáñez F and Fabra A. 2010. Phosphate-solubilizing peanut associated bacteria: screening for plant growth promoting activities. Plant and Soil 329: 421-431.
Vincent JM. 1970. A manual for the practical study of root nodule bacteria. IBM Handbook No. 15. Oxford: Blackwell Scientific Publications. Zhao L, Xu Y, Sun R, Deng Z, Yang W and Wei G. 2011. Identification 44and characterization of the endophytic plant growth promoter Bacillus cereus strain MQ23 isolated from Sophora alopecuroides root nodules. Brazilian Journal of Microbiology 42: 567-57.
